The immunocytochemical detection of cytochrome P-450 monooxygenase in the lungs of fetal, neonatal, and adult hamsters.
The immunocytochemical detection of cytochrome P-450 monooxygenase in the lungs of fetal, neonatal, and adult hamsters.
复制标题
胎儿、新生儿和成年仓鼠肺部细胞色素 P-450 单加氧酶的免疫细胞化学检测。
DOI：
10.1165/ajrcmb/2.6.493
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发表时间：
1990
影响因子：
6.4
通讯作者：
McDowell,EM
中科院分区：
文献类型：
--
作者：
Strum,JM;Ito,T;Philpot,RM;DeSanti,AM;McDowell,EM
材料和方法成年仓鼠研究在25只成年雌性仓鼠的肺上进行了初步研究，以发现一种能产生良好免疫化学结果和令人满意的光镜和电镜形态学的固定剂（18）。4%的多聚甲醛-0.1%的戊二醛在羧酸缓冲液中（19）产生了细胞色素P-450单加氧酶的强烈免疫过氧化物酶反应产物，因此它被选为本研究的固定剂。
Materials and MethodsAdult Hamster Studies Pilot studies to discover a fixative that would yield good immunochemical results and satisfactory morphology by light and electron microscopy were conducted on the lungs of 25 adult female hamsters (18). Four percent paraformaldehyde-0.1% glutaraldehyde in cacodylate buffer (19) yielded an intense immunoperoxidase reaction product for the cytochrome P-450 monooxygenase enzymes, and for this reason it was selected as the fixative for this investigation.