Correlation between Heart-type Fatty Acid-binding Protein Gene Polymorphism and mRNA Expression with Intramuscular Fat in Baicheng-oil Chicken.
Correlation between Heart-type Fatty Acid-binding Protein Gene Polymorphism and mRNA Expression with Intramuscular Fat in Baicheng-oil Chicken.
复制标题
白城油鸡心型脂肪酸结合蛋白基因多态性及mRNA表达与肌内脂肪的相关性
DOI：
10.5713/ajas.14.0886
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发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Wang Y;He J;Yang W;Muhantay G;Chen Y;Xing J;Liu J
本研究旨在探讨白城油鸡（BOC）心型脂肪酸结合蛋白（H-FABP）基因多态性和mRNA表达模式及其与肌内脂肪（IMF）含量的关系。试验共饲养720只鸡，其中乌鸡240只，乌鸡240只，白色白城油鸡240只。采用聚合酶链反应-单链构象多态性（PCR-SSCP）方法检测H-FABP基因第二外显子AA、AB、BB三种基因型。G939 A位点产生AA基因型，G956 A位点产生BB基因型。BBOC胸肌中AA基因型IMF含量显著高于AB（p = 0.0176），WBOC腿肌中AA基因型IMF含量显著高于AB（p = 0.0145）。G939 A位点可作为选择高IMF含量的胸肌和腿肌的遗传标记。在30、60、90和120 d通过实时PCR测定H-FABP的相对mRNA表达。随着年龄的增长，两种肌肉中的IMF含量显着增加。H-FABP mRNA在3种鸡肌肉中的表达量均随日龄的增加而显著降低。此外，观察到WBOC腿部肌肉中H-FABP丰度与IMF含量之间的显著负相关性（p = 0.035）。屠宰时，BOC胸肌和腿肌中H-FABP mRNA的表达量与IMF含量呈负相关。
This study aims to determine the polymorphism and mRNA expression pattern of the heart-type fatty acid-binding protein (H-FABP) gene and their association with intramuscular fat (IMF) content in the breast and leg muscles of Baicheng oil chicken (BOC). A total of 720 chickens, including 240 black Baicheng oil chicken (BBOC), 240 silky Baicheng oil chicken (SBOC), and 240 white Baicheng oil chicken (WBOC) were raised. Three genotypes of H-FABP gene second extron following AA, AB, and BB were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) strategy. The G939A site created AA genotype and G956A site created BB genotype. The content of IMF in AA genotype in breast muscle of BBOC was significantly higher than that of AB (p = 0.0176) and the genotype in leg muscle of WBOC was significantly higher than that of AB (p = 0.0145). The G939A site could be taken as genetic marker for higher IMF content selecting for breast muscle of BBOC and leg muscle of WBOC. The relative mRNA expression of H-FABP was measured by real-time PCR at 30, 60, 90, and 120 d. The IMF content significantly increased with age in both muscles. The mRNA expression level of H-FABP significantly decreased with age in both muscles of the three types of chickens. Moreover, a significant negative correlation between H-FABP abundance and IMF content in the leg muscles of WBOC (p = 0.035) was observed. The mRNA expression of H-FABP negatively correlated with the IMF content in both breast and leg muscles of BOC sat slaughter time.