Iris as a recipient tissue for pigment cells: Organized in vivo differentiation of melanocytes and pigmented epithelium derived from embryonic stem cells in vitro
Iris as a recipient tissue for pigment cells: Organized in vivo differentiation of melanocytes and pigmented epithelium derived from embryonic stem cells in vitro
复制标题
DOI：
10.1002/dvdy.21656
复制
发表时间：
2008-09-01
影响因子：
2.5
通讯作者：
Kunisada, Takahiro
中科院分区：
文献类型：
--
作者：
Aoki, Hitomi;Hara, Akira;Kunisada, Takahiro
将胚胎干（ES）细胞衍生的黑素细胞再生性移植到成人组织中，特别是包括毛囊或毛囊本身的皮肤中，通常是不可能的，而ES细胞衍生的色素上皮的再生性移植先前已有报道。我们研究了这两种色素细胞类型来源于ES细胞转移到虹膜后的体内分化。来自ES细胞的黑素细胞有效地整合到虹膜中并扩展以填充虹膜的基质层，就像从新生儿皮肤制备的那些一样。从胚胎干细胞或新生儿眼移植的色素上皮也被发现整合到虹膜中。这两种类型的再生色素细胞显示出正确的形态。再生色素上皮表达其功能标记物。功能性阻断黑素细胞发育所需的信号，可消除移植黑素细胞的分化。这些结果表明，成功地在体内再生转移的色素细胞诱导ES细胞在体外。
Regenerative transplantation of embryonic stem (ES) cell-derived melanocytes into adult tissues, especially skin that includes hair follicles or the hair follicle itself, generally not possible, whereas that of ES cell-derived pigmented epithelium was reported previously. We investigated the in vivo differentiation of these two pigment cell types derived from ES cells after their transfer into the iris. Melanocytes derived from ES cells efficiently integrated into the iris and expanded to fill the stromal layer of the iris, like those prepared from neonatal skin. Transplanted pigmented epithelium from either ES cells or the neonatal eye was also found to be integrated into the iris. Both types of these regenerated pigment cells showed the correct morphology. Regenerated pigment epithelium expressed its functional marker. Functional blocking of signals required for melanocyte development abolished the differentiation of transplanted melanocytes. These results indicate successful in vivo regenerative transfer of pigment cells induced from ES cells in vitro.