Applications of digital PCR in COVID-19 pandemic.
Applications of digital PCR in COVID-19 pandemic.
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DOI：
10.1002/viw.20200082
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Guo Y
中科院分区：
文献类型：
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作者：
Tan C;Fan D;Wang N;Wang F;Wang B;Zhu L;Guo Y
2019冠状病毒病（COVID-19）大流行导致公共卫生危机和全球恐慌。这种传染病是由一种名为严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的新型冠状病毒引起的。数字聚合酶链反应（dPCR）是一种新兴的核酸扩增技术，可以对核酸进行绝对定量，在SARS-CoV-2的检测中发挥着重要作用。本文介绍了dPCR的原理和优点，并综述了dPCR在COVID-19疫情中的应用，包括低拷贝数病毒的检测、病毒载量的测定、标准品的制备、环境中病毒浓度的监测、病毒突变的检测以及抗SARS-CoV-2药物的评价等。我们还讨论了dPCR在临床实践中的挑战。2019冠状病毒病（COVID-19）大流行导致公共卫生危机和全球恐慌。数字聚合酶链反应（dPCR）是一种新兴的核酸扩增技术，可对核酸进行绝对定量，在COVID-19病原体的检测中发挥着重要作用。本文介绍了dPCR的原理和优势，综述了dPCR在COVID-19疫情中的应用，并讨论了dPCR在临床实践中面临的挑战。
The coronavirus disease 2019 (COVID‐19) pandemic has led to a public health crisis and global panic. This infectious disease is caused by a novel coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Digital polymerase chain reaction (dPCR), which is an emerging nucleic acid amplification technology that allows absolute quantification of nucleic acids, plays an important role in the detection of SARS‐CoV‐2. In this review, we introduce the principle and advantages of dPCR, and review the applications of dPCR in the COVID‐19 pandemic, including detection of low copy number viruses, measurement of the viral load, preparation of reference materials, monitoring of virus concentration in the environment, detection of viral mutations, and evaluation of anti‐SARS‐CoV‐2 drugs. We also discuss the challenges of dPCR in clinical practice. The coronavirus disease 2019 (COVID‐19) pandemic has led to a public health crisis and global panic. Digital polymerase chain reaction (dPCR), which is an emerging nucleic acid amplification technology that allows absolute quantification of nucleic acids, plays an important role in the detection of the pathogen of COVID‐19. In this review, the authors introduce the principle and advantages of dPCR, review the applications of dPCR in the COVID‐19 pandemic, and discuss the challenges of dPCR in clinical practice.