Transcallosal circuitry revealed by blocking and disinhibiting callosal input in the cat.
Transcallosal circuitry revealed by blocking and disinhibiting callosal input in the cat.
复制标题
通过阻断和解除抑制猫的胼胝体输入来揭示经胼胝体回路。
DOI：
10.1017/s0952523800001553
复制
发表时间：
1994
影响因子：
1.9
通讯作者：
Diao,YC
中科院分区：
文献类型：
--
作者：
Sun,JS;Li,B;Ma,MH;Diao,YC
本研究的目的是获得定量测量胼胝体输入的影响，细胞在17/18区的边界区域，transcallosal轴突终止最密集。在右半球17/18区边缘区进行单细胞记录，而γ-氨基丁酸（GABA）或其拮抗剂荷包牡丹碱（bicuculline）应用于左半球的跨胼胝体投射区，以阻断或过度激活投射到记录部位神经元的细胞。结果表明，对侧半球给予GABA或荷包牡丹碱均能影响17/18区边缘细胞的视觉诱发反应。通过在左半球应用GABA来阻断经胼胝体输入减少了右半球51%的神经元的视觉诱发反应，并导致17%的神经元的反应幅度增加。通过应用荷包牡丹碱解除胼胝体输入的抑制，增加了40%的神经元的诱发活动，并减少了20%的神经元在右半球的反应幅度。GABA和荷包牡丹碱对某些细胞没有拮抗作用。32%的细胞只受一种药物给药的影响，13%的细胞在GABA和荷包牡丹碱给药后反应增加或减少。这项研究证明了通过胼胝体通路连接的神经元之间的复杂相互作用。一个模型的跨胼胝体电路提出了解释的结果。
The purpose of this study was to obtain quantitative measures of the influence of callosal input to cells at the area 17/18 border region where transcallosal axons terminate most densely. Single-cell recordings were performed at the area 17/18 border region of the right hemisphere, while gamma-aminobutyric acid (GABA) or its antagonist, bicuculline, were applied to the transcallosal projecting regions of the left hemisphere to either block or overactivate the cells which projected to the neurons at the recording site. The results showed that visually evoked responses of the cells at the area 17/18 border were affected by administration of GABA or bicuculline to the contralateral hemisphere. Blockade of transcallosal input by application of GABA in the left hemisphere diminished the visually evoked responses of 51% of the neurons in the right hemisphere, and led to an increase in response magnitude for 17% of the neurons. Disinhibition of transcallosal input by application of bicuculline increased the evoked activity of 40% of the neurons and diminished the response magnitude of 20% of the neurons in the right hemisphere. GABA and bicuculline failed to show antagonistic effects on some cells. Thirty-two percent of the cells were affected by only one type of drug administration, and 13% of the cells showed either an increase or a decrease in responses after both GABA and then bicuculline administration. This study demonstrated complex interactions between neurons connected by the transcallosal pathway. A model of the transcallosal circuitry was proposed to explain the results.