DETERMINATION OF LYMPHOCYTE DIVISION BY FLOW-CYTOMETRY
DETERMINATION OF LYMPHOCYTE DIVISION BY FLOW-CYTOMETRY
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DOI：
10.1016/0022-1759(94)90236-4
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发表时间：
1994-05-02
影响因子：
2.2
通讯作者：
PARISH, CR
中科院分区：
文献类型：
--
作者：
LYONS, AB;PARISH, CR
目前可用于确定细胞分裂的技术能够显示一次或最多有限次数的细胞分裂。存在其他方法，其中I：量化整体分裂，但不告诉任何关于单个细胞的分裂历史。在这里，我们提出了一种新的技术，其中细胞内的荧光标记在细胞分裂时在子细胞之间平均分配。该技术适用于体外细胞分裂以及过继转移细胞的体内分裂，并且可以使用流式细胞术解析多个连续代。该标记是荧光素衍生的，允许与藻红蛋白或其他相容的荧光染料缀合的单克隆抗体用于对分裂细胞进行免疫表型分析。
Techniques currently available for determining cell division are able to show one or, at best, a limited number of cell divisions. Other methods exist which I:an quantify overall division, but tell nothing about the division history of individual cells. Here we present a new technique in which an intracellular fluorescent label is divided equally between daughter cells upon cell division. The technique is applicable to in vitro cell division, as well as in vivo division of adoptively transferred cells, and can resolve multiple successive generations using flow cytometry. The label is fluorescein derived, allowing monoclonal antibodies conjugated to phycoerythrin or other compatible fluorochromes to be used to immunophenotype the dividing cells.