Combination of micelle collapse and field-amplified sample stacking in capillary electrophoresis for determination of trimethoprim and sulfamethoxazole in animal-originated foodstuffs
Combination of micelle collapse and field-amplified sample stacking in capillary electrophoresis for determination of trimethoprim and sulfamethoxazole in animal-originated foodstuffs
复制标题
结合毛细管电泳中的胶束崩塌和场放大样品堆积来测定动物源性食品中的甲氧苄啶和磺胺甲恶唑
DOI：
10.1016/j.foodchem.2016.09.118
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发表时间：
2017-03-15
期刊：
影响因子：
8.8
通讯作者：
Yang, Chunli
中科院分区：
文献类型：
--
作者：
Liu, Lihong;Wan, Qian;Yang, Chunli
采用胶束-溶剂堆积（MSS）和场放大样品堆积（FASS）相结合的在线预富集方法，建立了甲氧苄啶（TMP）和磺胺甲恶唑（SMZ）的毛细管区带电泳（CZE）分析方法。优化的实验条件为：（1）样品基质：10.0 mM SDS-5%（v/v）甲醇;（2）捕获液：35 mM H3 PO 4 -60%乙腈（CH 3CN）;（3）运行缓冲液：30 mM Na 2 HPO 4（pH = 7.3）;（4）样品溶液体积，168 nL; TS体积，168 nL;和（5）9 kV电压，214 nm UV检测。在优化条件下，SMZ和TMP的检测限（LOD）分别为7.7和8.5 ng/mL，分别是典型进样的301和329倍。对乳制品、鸡蛋、蜂蜜等动物性食品中TMP和SMZ的含量进行了分析。回收率为80-104%，相对标准偏差为0.5- 5.4%。(C)2016爱思唯尔有限公司版权所有
An on-line preconcentration method combining micelle to solvent stacking (MSS) with field-amplified sample stacking (FASS) was employed for the analysis of trimethoprim (TMP) and sulfamethoxazole (SMZ) by capillary zone electrophoresis (CZE). The optimized experimental conditions were as followings: (1) sample matrix, 10.0 mM SDS-5% (v/v) methanol; (2) trapping solution (TS), 35 mM H3PO4-60% acetonitrile (CH3CN); (3) running buffer, 30 mM Na2HPO4 (pH = 7.3); (4) sample solution volume, 168 nL; TS volume, 168 nL; and (5) 9 kV voltage, 214 nm UV detection. Under the optimized conditions, the limits of detection (LODs) for SMZ and TMP were 7.7 and 8.5 ng/mL, and they were 301 and 329 times better compared to a typical injection, respectively. The contents of TMP and SMZ in animal foodstuffs such as dairy products, eggs and honey were analyzed, too. Recoveries of 80-104% were acquired with relative standard deviations of 0.5-5.4%. (C) 2016 Elsevier Ltd. All rights reserved.