Bisphosphonates induce apoptosis in human myeloma cell lines: a novel anti-tumour activity
Bisphosphonates induce apoptosis in human myeloma cell lines: a novel anti-tumour activity
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DOI：
10.1046/j.1365-2141.1997.2713086.x
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发表时间：
1997-09-01
影响因子：
6.5
通讯作者：
Croucher, PI
中科院分区：
文献类型：
--
作者：
Shipman, CM;Rogers, MJ;Croucher, PI
双膦酸盐广泛用于多种代谢性和肿瘤诱导的骨病，包括多发性骨髓瘤，以防止骨吸收。最近的报道表明，双膦酸盐治疗可能与患者生存率的增加有关，这提高了这些化合物可能对肿瘤细胞有直接作用的可能性。我们在体外研究了氯膦酸盐、帕米膦酸盐和YM175是否能直接影响人骨髓瘤细胞系U266-B1、JIN-3和HS-Sultan。采用流式细胞术检测双膦酸盐处理对细胞数量和细胞周期进展的影响。双膦酸盐诱导人骨髓瘤细胞系凋亡的能力是根据细胞核形态和DNA片段的变化来确定的。帕米膦酸盐和更强效的双膦酸盐YM175显著降低细胞数量(P
Bisphosphonates are in widespread use to prevent bone resorption in a number of metabolic and tumour-induced bone diseases including multiple myeloma. Recent reports suggest that bisphosphonate treatment may be associated with an increase in patient survival, raising the possibility that these compounds may have a direct effect on the tumour cells. We have investigated whether the bisphosphonates clodronate, pamidronate and YM175 can directly affect the human myeloma cell lines U266-B1, JIN-3 and HS-Sultan in vitro. The effect of bisphosphonate treatment on cell number and cell cycle progression was examined using now cytometry. The ability of bisphosphonates to induce apoptosis in human myeloma cell lines was determined on the basis of changes in nuclear morphology and of DNA fragmentation. Pamidronate and the more potent bisphosphonate, YM175, significantly decreased cell number (P