A drug screening toolkit based on the -1 ribosomal frameshifting of SARS-CoV-2
A drug screening toolkit based on the -1 ribosomal frameshifting of SARS-CoV-2
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DOI：
10.1016/j.heliyon.2020.e04793
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发表时间：
2020-08-01
期刊：
影响因子：
4
通讯作者：
Fu, Xinyuan
中科院分区：
文献类型：
--
作者：
Chen, Yanqiong;Tao, Huan;Fu, Xinyuan
-1核糖体移码对于SARS-CoV-2中开放阅读框架(ORF)1b的翻译至关重要。ORF1b的产物参与病毒复制。因此，改变移码频率会降低病毒的存活率。本研究旨在成功开发一种用于筛选抗病毒药物的工具包。最后，对FDA批准的药物文库进行了筛选，结果表明，异烟肼和(-)-石杉碱甲在体外能很好地改变SARS-CoV-2的核糖体移码。
The -1 ribosomal frameshifting is vital for the translation of the open reading frame (ORF)1b in SARS-CoV-2. The products of ORF1b participate in viral replication. Therefore, changing the frameshift frequency reduces the survival of the virus. This study aimed to successfully develop a toolkit for screening antiviral drugs. Finally, the FDA-approved drug library was screened, revealing that ivacaftor and (-)-Huperzine A worked well in changing the -1 ribosomal frameshifting of SARS-CoV-2 in vitro.