Characteristics of Streptococcus mutans strains lacking the MazEF and RelBE toxin-antitoxin modules
Characteristics of Streptococcus mutans strains lacking the MazEF and RelBE toxin-antitoxin modules
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DOI：
10.1016/j.femsle.2005.09.045
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发表时间：
2005-12-15
影响因子：
2.1
通讯作者：
Burne, RA
中科院分区：
文献类型：
--
作者：
Lemos, JAC;Brown, TA;Burne, RA
在变形链球菌中鉴定出两对基因与大肠杆菌的 relBE 和 mazEF 毒素-抗毒素 (TA) 模块相似。 mazEF 和 relBE 的转录受到氨基酸饥饿的抑制，relBE 的表达受到低 pH 的抑制。缺乏 MazF、RelE 或两种毒素 (MRT1) 的突变体在肉汤培养基中生长，并与亲本一样形成生物膜。 MRT1 生物膜群体比亲本或单一突变体更能抵抗酸杀伤。 MRT1 在葡萄糖和菊粉上生长期间还表现出更长的双轴，并表现出降低的磷酸烯醇丙酮酸：糖磷酸转移酶活性。这是第一份证明 TA 模块在革兰氏阳性菌中的生理作用的报告。 (c) 2005 年欧洲微生物学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Two pairs of genes were identified in Streptococcus mutans with similarity to relBE and mazEF toxin-antitoxin (TA) modules of Escherichia coli. Transcription of mazEF and relBE was repressed by amino acid starvation, and relBE expression was repressed by low pH. Mutants lacking MazF, RelE, or both toxins (MRT1) grew in broth media and formed biofilms as well as the parent. Biofilm populations of MRT1 were more resistant to acid killing than the parent or single mutants. MRT1 also exhibited a longer diauxie during growth on glucose and inulin and displayed decreased phosphoenolpyruvate: sugar phosphotransferase activity. This is the first report that demonstrates a physiological role for TA modules in Gram-positive bacteria. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.