Microbubble persistence in the microcirculation during ischemia/reperfusion and inflammation is caused by integrin- and complement-mediated adherence to activated leukocytes
Microbubble persistence in the microcirculation during ischemia/reperfusion and inflammation is caused by integrin- and complement-mediated adherence to activated leukocytes
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DOI：
10.1161/01.cir.101.6.668
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发表时间：
2000-02-15
期刊：
影响因子：
37.8
通讯作者：
Ley, K
中科院分区：
文献类型：
--
作者：
Lindner, JR;Coggins, MP;Ley, K
背景-用于超声造影的白蛋白微泡在缺血/再灌注（I-R）后持续存在于心肌微循环中。造成这种现象的机制尚不清楚，方法和结果-在静脉内注射由白蛋白、阴离子脂质或阳离子脂质组成的荧光素标记的微泡期间，在12只野生型小鼠中进行外置提睾肌微循环的活体显微镜检查，8只小鼠在基线和I-R 30 - 90分钟后注射，4只小鼠在阴囊内注射肿瘤坏死因子-α（TNF-α）2小时后注射。基线时微泡粘附并不常见（
Background-Albumin microbubbles that are used for contrast echocardiography persist within the myocardial microcirculation after ischemia/reperfusion (I-R). The mechanism responsible for this phenomenon is unknown,Methods and Results-Intravital microscopy of the microcirculation of exteriorized cremaster muscle was performed in 12 wild-type mice during intravenous injections of fluorescein-labeled microbubbles composed of albumin, anionic lipids, or cationic lipids, Injections were performed at baseline and after 30 to 90 minutes of I-R in 8 mice and 2 hours after intrascrotal tumor necrosis factor-alpha (TNF-alpha) in 4 mice. Microbubble adherence at baseline was uncommon (