MOLECULAR CHANGES IN THE MEMBRANES OF MOUSE ERYTHROID-CELLS ACCOMPANYING DIFFERENTIATION
MOLECULAR CHANGES IN THE MEMBRANES OF MOUSE ERYTHROID-CELLS ACCOMPANYING DIFFERENTIATION
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DOI：
10.1016/0092-8674(79)90196-x
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发表时间：
1979-01-01
期刊：
影响因子：
64.5
通讯作者：
SINGER, SJ
中科院分区：
文献类型：
--
作者：
GEIDUSCHEK, JB;SINGER, SJ
小鼠成红细胞向成熟红细胞的发育伴随着这些细胞质膜的组成和性质的变化。利用双荧光技术，凝集素受体和血影蛋白的分布在这些细胞的膜上的同时测定。随着分化的进行，膜中凝集素受体的横向迁移率降低，并且这伴随着与膜相关的血影蛋白浓度的增加。血影蛋白的最显著浓度发生在晚期成红细胞的去核过程中。可以观察到血影蛋白完全分离到初始网织红细胞中，以及之前观察到的伴刀豆球蛋白[Con] A受体富集到突出细胞核周围的质膜中。在这些和其他观察的基础上，探讨了成红细胞去核的可能分子机制和血影蛋白在红系细胞膜蛋白流动性调节中的作用。
The development of the mouse erythroblast to a mature erythrocyte was accompanied by changes in the composition and properties of the plasma membranes to these cells. Using double fluorescence techniques, the distribution of lectin receptors and spectrin on the membranes of these cells were simultaneously determined. The lateral mobility of the lectin receptors in the membranes decreased as differentiation proceeded and this was accompanied by an increasing concentration of spectrin associated with the membranes. The most significant concentration of spectrin occurred during the enucleation of the late erythroblast. A complete segregation of the spectrin to the incipient reticulocyte could be observed, as well as a previously observed enrichment of receptors for concanavalin [Con] A into the plasma membrane surrounding the extruding nucleus. On the basis of these and other observations, the possible molecular mechanisms involved in erythroblast enucleation and the role of spectrin in the regulation of protein mobility in erythroid cell membranes were explored.