Expanding the scope of RNA catalysis.
Expanding the scope of RNA catalysis.
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扩大RNA催化的范围。
DOI：
10.1126/science.8009223
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Schultz,PG
中科院分区：
文献类型：
--
作者：
Prudent,JR;Uno,T;Schultz,PG
过渡态理论的基本概念在过去被用来从免疫系统中庞大的抗体分子库中产生高选择性的催化剂。这些相同的想法被用来从一个大的RNA库中分离出一个RNA分子，它催化桥连联苯的异构化。该反应符合米氏动力学，催化速率常数（kcat）为2.8 × 10- 5/min，米氏常数（Km）为542 μM，该反应被平面过渡态类似物竞争性抑制，抑制常数（Ki）为0.77 μM.这种方法可以提供一个通用的策略，扩大RNA催化的范围超出那些反应，其中的底物是核酸或核酸衍生物。
The basic notions of transition state theory have been exploited in the past to generate highly selective catalysts from the vast library of antibody molecules in the immune system. These same ideas were used to isolate an RNA molecule, from a large library of RNAs, that catalyzes the isomerization of a bridged biphenyl. The RNA-catalyzed reaction displays Michaelis-Menten kinetics with a catalytic rate constant (kcat) of 2.8 × 10-5per minute and a Michaelis constant (Km) of 542 μM; the reaction is competitively inhibited by the planar transition state analog with an inhibition constant (Ki) value of ∼7 μM. This approach may provide a general strategy for expanding the scope of RNA catalysis beyond those reactions in which the substrates are nucleic acids or nucleic acid derivatives.