UTILIZATION OF SODIUM-DEPENDENT HIGH AFFINITY CHOLINE UPTAKE INVITRO AS A MEASURE OF ACTIVITY OF CHOLINERGIC NEURONS INVIVO
UTILIZATION OF SODIUM-DEPENDENT HIGH AFFINITY CHOLINE UPTAKE INVITRO AS A MEASURE OF ACTIVITY OF CHOLINERGIC NEURONS INVIVO
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DOI：
10.1016/0024-3205(75)90174-5
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发表时间：
1975-01-01
期刊：
影响因子：
6.1
通讯作者：
KUHAR, MJ
中科院分区：
文献类型：
--
作者：
ATWEH, S;SIMON, JR;KUHAR, MJ
体内胆碱能神经元活性的改变伴随着体外na依赖性高亲和力胆碱摄取的平行变化。这部分胆碱摄取可能对乙酰胆碱[ACh]的合成有调节作用。Na+依赖性高亲和力胆碱摄取可作为体内胆碱能活性相对状态的量度。一些药物被用于改变乙酰胆碱的转换和释放（两者都是体内胆碱能活性的测量指标），随后研究了体外Na+依赖性高亲和力胆碱摄取。给药戊巴比妥，水合氯醛，吗啡，毒豆碱，德尔塔。9-tetrahydrocannabinol [.DELTA。9-THC]， hololium -3和oxotremorine，这些药物减少乙酰胆碱的转化和释放，导致胆碱摄取减少。相反，使用戊四唑、阿托品、东莨菪碱和氟哌啶醇这些增加乙酰胆碱转换和释放的药物，在体外引起胆碱摄取增加。Na+依赖性高亲和力胆碱摄取显然可以作为体内胆碱能神经元活性的相对测量。
Alterations of activity of cholinergic neurons in vivo are followed by parallel changes in Na-dependent high affinity choline uptake in vitro. This portion of choline uptake may be regulatory in the synthesis of acetylcholine [ACh]. The Na+-dependent high affinity choline uptake may be used as a measure of the relative state of cholinergic activity in vivo. A number of drugs were administered to alter turnover and release of ACh (both are measures of cholinergic activity in vivo), and subsequently Na+-dependent high affinity choline uptake in vitro was examined. Administration of pentobarbital, chloral hydrate, morphine, physostigmine, .DELTA.9-tetrahydrocannabinol [.DELTA.9-THC], hemicholinium-3 and oxotremorine, drugs which decrease ACh turnover and release, caused a reduction in choline uptake. Conversely, administration of pentylenetetrazol, atropine, scopolamine, and haloperidol, drugs which increase ACh turnover and release, caused an increase in choline uptake in vitro. Na+ dependent high affinity choline uptake can apparently be used as a relative measure of the activity of cholinergic neurons in vivo.