An extended epidermal response heals cutaneous wounds in the absence of a hair follicle stem cell contribution
An extended epidermal response heals cutaneous wounds in the absence of a hair follicle stem cell contribution
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DOI：
10.1038/sj.jid.5701178
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发表时间：
2008-05-01
影响因子：
6.5
通讯作者：
Headon, Denis J.
中科院分区：
文献类型：
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作者：
Langton, Abigail K.;Herrick, Sarah E.;Headon, Denis J.
已观察到毛囊对表皮愈合有重要的细胞贡献，毛囊中干细胞衍生细胞的迁移有助于伤口的再上皮化。然而，这种毛囊输入的功能需求尚不清楚。在此，我们对尾部无任何毛囊发育的突变小鼠的角质形成细胞干细胞状态进行了表征，并分析了表皮伤口愈合速率和机制随之发生的改变。在分析胚胎皮肤中的干细胞行为时，我们发现，在毛囊形成之前，外胚层中克隆形成性角质形成细胞相对较多。然而，到出生时，它们在毛囊间表皮中的频率急剧下降，此时大多数干细胞存在于毛囊内。我们发现，在没有毛囊的情况下，皮肤伤口愈合时再上皮化会严重延迟。这种延迟之后是活化表皮区域的扩大，超过正常有毛皮肤中的情况，然后伤口适当闭合。
Hair follicles have been observed to provide a major cellular contribution to epidermal healing, with emigration of stem-derived cells from the follicles aiding in wound reepithelialization. However, the functional requirements for this hair follicle input are unknown. Here we have characterized the keratinocyte stem cell status of mutant mice that lack all hair follicle development on their tail, and analyzed the consequent alterations in epidermal wound healing rate and mechanisms. In analyzing stem cell behavior in embryonic skin we found that clonogenic keratinocytes are relatively frequent in the ectoderm prior to hair follicle formation. However, their frequency in the interfollicular epidermis drops sharply by birth, at which time the majority of stem cells are present within the hair follicles. We find that in the absence of hair follicles cutaneous wounds heal with an acute delay in reepithelialization. This delay is followed by expansion of the region of activated epidermis, beyond that seen in normal haired skin, followed by appropriate wound closure.