Retropseudogenes derived from the human Ro/SS-A autoantigen-associated hY RNAs.
Retropseudogenes derived from the human Ro/SS-A autoantigen-associated hY RNAs.
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DOI：
10.1093/nar/gki504
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发表时间：
2005
影响因子：
14.9
通讯作者：
Boire G
中科院分区：
文献类型：
--
作者：
Perreault J;Noël JF;Brière F;Cousineau B;Lucier JF;Perreault JP;Boire G
我们报告了来自四个人Y（hY）RNA（Ro/SS-A自身抗原的组分）的966个假基因在人类基因组中的表征。大约95%的Y RNA假基因位于黑猩猩和人类染色体上的相应位置。相反，小鼠中的Y假基因既不常见，又存在于不同的基因组区域。除了这种啮齿动物/灵长类动物的差异，相对于hY基因的hY假基因的保守性表明，它们发生在啮齿动物/灵长类动物的分歧。hY假基因的侧翼区域包含令人信服的证据，参与了L1反转录转座机制。虽然发现Alu元件与大多数hY假基因非常接近，但这些不是嵌合逆转录基因。特异性影响Ro60蛋白结合的hY RNA转录物中的点突变可能有助于它们选择直接反式反转录转座。这代表了通过L1反转录转座机制选择用于其基因组整合的特定RNA的新要求。超过40%的hY假基因位于蛋白质编码基因的内含子区域。考虑到已知结合hY RNA子集的蛋白质的功能，hY假基因构成了一类新的L1依赖性非自主逆转录元件，可能参与基因表达的转录后调控。
We report the characterization in the human genome of 966 pseudogenes derived from the four human Y (hY) RNAs, components of the Ro/SS-A autoantigen. About 95% of the Y RNA pseudogenes are found in corresponding locations on the chimpanzee and human chromosomes. On the contrary, Y pseudogenes in mice are both infrequent and found in different genomic regions. In addition to this rodent/primate discrepancy, the conservation of hY pseudogenes relative to hY genes suggests that they occurred after rodent/primate divergence. Flanking regions of hY pseudogenes contain convincing evidence for involvement of the L1 retrotransposition machinery. Although Alu elements are found in close proximity to most hY pseudogenes, these are not chimeric retrogenes. Point mutations in hY RNA transcripts specifically affecting binding of Ro60 protein likely contributed to their selection for direct trans retrotransposition. This represents a novel requirement for the selection of specific RNAs for their genomic integration by the L1 retrotransposition machinery. Over 40% of the hY pseudogenes are found in intronic regions of protein-coding genes. Considering the functions of proteins known to bind subsets of hY RNAs, hY pseudogenes constitute a new class of L1-dependent non-autonomous retroelements, potentially involved in post-transcriptional regulation of gene expression.
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DOI：
10.1073/pnas.0831042100
发表时间：
2003-04-29
影响因子：
11.1
作者：
Brouha, B;Schustak, J;Kazazian, HH
通讯作者：
Kazazian, HH
影响因子：
30.8
作者：
Dewannieux, M;Esnault, C;Heidmann, T
通讯作者：
Heidmann, T
影响因子：
5.6
作者：
Fouraux, MA;Bouvet, P;Pruijn, GJM
通讯作者：
Pruijn, GJM
影响因子：
4.5
作者：
Green, CD;Long, KS;Wolin, SL
通讯作者：
Wolin, SL
影响因子：
5.8
作者：
Grover, D;Mukerji, M;Brahmachari, SK
通讯作者：
Brahmachari, SK