IgE antibody up-regulates high affinity IgE binding on murine bone marrow-derived mast cells
IgE antibody up-regulates high affinity IgE binding on murine bone marrow-derived mast cells
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DOI：
10.1016/0165-2478(96)02599-0
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发表时间：
1996-09-01
影响因子：
4.4
通讯作者：
MacGlashan, D
中科院分区：
文献类型：
--
作者：
Hsu, C;MacGlashan, D
我们检测了来自小鼠骨髓的3周大的阿尔新蓝阳性细胞(可能是肥大细胞)的Fc epsilon RI的表达。用免疫球蛋白E(IgE)抗体(抗DNP IgE)间接致敏细胞，并用流式细胞仪检测结合的IgE抗体水平，我们发现，延长培养时间(1-5天)，加入IgE，而不加Ig G，总受体密度增加6+/-1.9倍。在同一时期，对抗原的组胺释放(DNP-HSA)增加了大约6倍，而细胞对凝血酶或离子霉素的反应保持不变。最大的上调发生在培养的前2天。用2.462检测Fc-Gamma RII/RIII，我们没有检测到该受体的任何上调。致敏后培养1h没有导致细胞表面IgE的任何丢失，这表明与Fc epsilon RI的预期类似的是一个相当高的亲和力结合。此上调作用可被2mU g/ml放线菌亚胺完全抑制。这些数据表明，IgE能够诱导显著的蛋白质合成，依赖于肥大细胞/嗜碱性粒细胞上自身高亲和力受体的上调。
We have examined 3-week-old alcian blue positive cells (putatively mast cells) derived from mouse bone marrow for their expression of Fc epsilon RI. Using an indirect method of sensitizing the cells with immunoglobulin E (IgE) antibody (anti-DNP IgE) and detecting the level of bound IgE antibody by flow cytometry, we found that prolonged culture (1-5 days) with IgE, but not IgG, increased the total receptor density 6 +/- 1.9 fold. During the same period, histamine release in response to antigen (DNP-HSA) increased approximately 6-fold while the cell's response to either thrombin or ionomycin remained constant. The greatest up-regulation occurred in the first 2 days of culture. Using 2.462 to detect Fc gamma RII/RIII, we could not detect any up-regulation of this receptor. Culturing the cells for 1 h after sensitization did not result in any loss of cell surface IgE, suggesting a reasonably high affinity binding similar to that expected for Fc epsilon RI. This up-regulation was completely inhibited by co-culture with 2 mu g/ml cycloheximide. These data suggest that IgE is capable of inducing a significant, protein synthesis, dependent up-regulation of its own high affinity receptor on mast cells/basophils.