Phospholipid abnormalities in postmortem schizophrenic brains detected by 31P nuclear magnetic resonance spectroscopy:: a preliminary study
Phospholipid abnormalities in postmortem schizophrenic brains detected by 31P nuclear magnetic resonance spectroscopy:: a preliminary study
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DOI：
10.1016/s0925-4927(01)00081-6
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发表时间：
2001-05-30
影响因子：
2.3
通讯作者：
Karson, CN
中科院分区：
文献类型：
--
作者：
Komoroski, RA;Pearce, JM;Karson, CN
据推测，精神分裂症是由于磷脂（PL）组成和代谢变化导致的细胞膜异常引起的。我们使用高分辨率体外 P-31 核磁共振 (NMR) 来表征 4 名精神分裂症患者和 5 名对照者死后痘痘的左额皮质（灰质）中的 PL。高分辨率 31P NMR 谱是在有机溶剂系统中获得的，以解析 PL 类别（头基），并在胆酸钠水分散系统中获得，以解析磷脂酰胆碱 (PC) 分子种类。包括主要 PC 分子种类和磷脂酰肌醇 (PI) 在内的多变量分析显示，精神分裂症对照者之间存在显着差异。特定相互作用分析表明，精神分裂症组的 PI 显着高于对照组。对于其他单独的 PL 类别或由甘油上 sn-1 位置的连接类型确定的单独 PL 亚类，两组之间没有差异。精神分裂症患者的总 PL 含量有高于对照组的趋势。没有证据表明精神分裂症患者溶血磷脂酰胆碱或溶血磷脂酰乙醇胺升高。精神分裂症组代表具有一个饱和和一个不饱和酰基链或两个双键酰基链的分子种类的PC峰的强度高于对照组。尽管这些结果与之前的研究并不完全一致，但它们支持了精神分裂症患者大脑中发生PL异常以及脂肪酸代谢可能异常的观点。 (C) 2001 Elsevier Science Ireland Ltd. 保留所有权利。
It has been hypothesized that schizophrenia arises from cell membrane abnormalities due to changes in phospholipid (PL) composition and metabolism. We have used high resolution, in vitro P-31 nuclear magnetic resonance (NMR) to characterize the PLs in left frontal cortex (gray matter) of postmortem blain from four schizophrenics and five controls. High resolution 31P NMR spectra were obtained in an organic-solvent system to resolve PL classes (headgroups) and in a sodium-cholate, aqueous dispersion system to resolve phosphatidylcholine (PC) molecular species. Multivariate analysis which included the major PC molecular species and phosphatidylinositol (PI) showed a significant difference between schizophrenics rind controls. Analysis of specific interactions showed that the PI was significantly higher in the schizophrenic group than in the control group. There were no differences between the two groups for other individual PL classes, or for individual PL subclasses determined by the linkage type at the sn-l position on glycerol. There was a trend for total PL content to be higher in schizophrenics than in controls. There was no evidence for elevated lysophosphatidylcholine or lysophosphatidylethanolaminc in schizophrenia. The intensity of the PC peak representing molecular species with one saturated and one unsaturated tone or two double bonds) acyl chain was higher for the schizophrenic group than for the control group. Although these results: are not in complete agreement with previous studies, they support the idea that PL abnormalities occur in the brain in schizophrenia and that fatty acid metabolism may be abnormal. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.