Effects of Anaerobiosis on Chlorophyll Fluorescence Yield in Spinach (Spinacia oleracea) Leaf Discs
Effects of Anaerobiosis on Chlorophyll Fluorescence Yield in Spinach (Spinacia oleracea) Leaf Discs
复制标题
厌氧对菠菜叶盘叶绿素荧光产量的影响
作者：
G. Harris;U. Heber
菠菜叶盘在黑暗厌氧环境中培养时，其叶绿素荧光产量较好氧对照有较大幅度的提高。偶尔，变暗的厌氧样品的荧光产量接近最大荧光的80%。在厌氧潜育期内，还诱导出了在叶盘中表现出弱光介导的叶绿素荧光诱导现象。这涉及到荧光产量的快速和缓慢增加，随后是缓慢的猝灭。这可能是由低至400[亩]W·m~(-2)的光照水平引起的。依赖于厌氧的叶绿素荧光产量的增加可以被远红光、O2或饱和的白光脉冲所缓解。结果表明，叶绿素荧光产量的增加主要是由于叶绿体对苯二酚库的暗还原和再氧化使其松弛所致。在厌氧条件下，变暗的分离叶绿体没有表现出荧光产量的增加。只有当加入二硫苏糖醇或二亚硫酸盐时，荧光才缓慢增强。
When spinach (Spinacia oleracea) leaf discs were incubated in a dark anaerobic environment, the chlorophyll fluorescence yield was much increased relative to the aerobic control. Occasionally, the fluorescence yield of the darkened anaerobic samples approached 80% of the maximum fluorescence. The anaerobic incubation period also induced in a leaf disc the capacity to exhibit a low light-mediated chlorophyll fluorescence induction phenomenon. This involved a rapid and slow increase in fluorescence yield, followed by a slow quenching. This could be induced by light levels as low as 400 [mu]W m-2. The anaerobic-dependent increase in chlorophyll fluorescence yield could be relaxed by either far-red light, O2, or a saturating pulse of white light. It was concluded that the anaerobic-dependent increase in chlorophyll fluorescence yield was due to a dark reduction of the plastoquinone pool and its relaxation by reoxidation. Darkened isolated chloroplasts did not exhibit a fluorescence yield increase under anaerobic conditions. Fluorescence slowly increased only when dithiothreitol or dithionite was added.