Salsolinol is present in the bovine posterior pituitary gland and stimulates the release of prolactin both in vivo and in vitro in ruminants
Salsolinol is present in the bovine posterior pituitary gland and stimulates the release of prolactin both in vivo and in vitro in ruminants
复制标题
DOI：
10.1016/j.domaniend.2006.12.003
复制
发表时间：
2008-02-01
影响因子：
2.1
通讯作者：
Nagy, G. M.
中科院分区：
文献类型：
--
作者：
Hashizume, T.;Shida, R.;Nagy, G. M.
本研究的目的是确定是否salsolinol（SAL），多巴胺相关的化合物，存在于牛垂体后叶（PP）腺，并阐明SAL对泌乳素（PRL）分泌的反刍动物。采用高压液相色谱-电化学检测法（HPLC-EC）检测牛PP腺提取液中的SAL。单次静脉内（i. v.）注射SAL（5和10 mg/kg体重）可显著促进山羊泌乳素的释放，并呈剂量依赖性（P < 0.05）。血浆PRL水平在注射后10分钟达到峰值，然后在60-80分钟逐渐恢复到基础值。PRL释放模式与舒必利（多巴胺受体拮抗剂）相似。脑室内（i. c. v.）注射1 mg SAL对泌乳素的释放无显著影响，而注射5 mg SAL对泌乳素的释放有显著促进作用（P < 0.05），其峰值出现在注射后30-40 min。10（-6）M和10（-5）M的SAL能显著刺激培养的牛垂体前叶细胞分泌PRL（P < 0.05）。综上所述，我们的数据清楚地表明，SAL存在于反刍动物PP腺提取物中，并且在体内和体外都具有PRL释放活性。因此，这种内源性化合物是先前在牛PP腺提取物中检测到的具有PRL释放活性的因子的强有力候选物。(c)2007爱思唯尔公司All rights reserved.
The aims of the present study were to determine whether salsolinol (SAL), a dopamine-related compound, is present in the bovine posterior pituitary (PP) gland, and to clarify the effect of SAL on the secretion of prolactin (PRL) in ruminants. SAL was detected in extract of bovine PP gland using high-pressure liquid chromatography with electrochemical detection (HPLC-EC). A single intravenous (i.v.) injection of SAL (5 and 10 mg/kg body weight) significantly and dose-dependently stimulated the release of PRL in goats (P < 0.05). Plasma PRL levels reached a peak 10 min after the injection, then gradually returned to basal values in 60-80 min. The PRL-releasing pattern was similar to that in response to sulpiride (a dopamine receptor antagonist). The intracerebroventricular (i.c.v.) injection of I mg of SAL had no significant effect on the release of PRL in calves, however, 5 mg significantly stimulated the release (P < 0.05) with peak values reached 30-40 min after the injection. Moreover, SAL significantly stimulated the release of PRL from cultured bovine anterior pituitary cells at doses of 10(-6) and 10(-5) M, compared to control cells (P < 0.05). Taken together, our data clearly show that SAL is present in extract of the PP gland of ruminants, and has PRL-releasing activity both in vivo and in vitro. Therefore, this endogenous compound is a strong candidate for the factor having PRL-releasing activity that has been previously detected in extract of the bovine PP gland. (c) 2007 Elsevier Inc. All rights reserved.