TRANVIA (TVA) facilitates cellulose synthase trafficking and delivery to the plasma membrane.
TRANVIA (TVA) facilitates cellulose synthase trafficking and delivery to the plasma membrane.
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TRAVIA (TVA) 促进纤维素合酶运输并递送至质膜。
DOI：
10.1073/pnas.2021790118
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发表时间：
2021
影响因子：
11.1
通讯作者：
Ehrhardt,DavidW
中科院分区：
文献类型：
--
作者：
Vellosillo,Tamara;Dinneny,JoséR;Somerville,ChrisR;Ehrhardt,DavidW
纤维素由纤维素合酶 (CESA) 复合物 (CSC) 在质膜上合成，这些复合物在高尔基体中组装并通过反式高尔基体网络 (TGN) 室分泌到质膜。然而，引导 CSC 通过分泌系统并将其递送至质膜的分子机制尚不清楚。在这里，我们鉴定了一个未表征的基因，TRANVIA（TVA），它与原代细胞壁合成所需的CESA基因在转录上共同调节。 Thetvamutant 对纤维素合成抑制剂表现出增强的敏感性；降低纤维素含量；与野生型相比，CSC 的动态、密度和质膜分泌有缺陷。 TVA 是一种功能未知的植物特异性蛋白质，可在至少两个不同的细胞内区室中检测到：由 TGN 标记标记的细胞器和将 CSC 传递到质膜的较小区室。总之，我们的数据表明，TVA 通过促进 TGN 的退出和/或 CSC 分泌囊泡与质膜的相互作用来促进 CSC 向质膜的运输。
Cellulose is synthesized at the plasma membrane by cellulose synthase (CESA) complexes (CSCs), which are assembled in the Golgi and secreted to the plasma membrane through thetrans-Golgi network (TGN) compartment. However, the molecular mechanisms that guide CSCs through the secretory system and deliver them to the plasma membrane are poorly understood. Here, we identified an uncharacterized gene,TRANVIA(TVA), that is transcriptionally coregulated with theCESAgenes required for primary cell wall synthesis. Thetvamutant exhibits enhanced sensitivity to cellulose synthesis inhibitors; reduced cellulose content; and defective dynamics, density, and secretion of CSCs to the plasma membrane as compared to wild type. TVA is a plant-specific protein of unknown function that is detected in at least two different intracellular compartments: organelles labeled by markers for the TGN and smaller compartments that deliver CSCs to the plasma membrane. Together, our data suggest that TVA promotes trafficking of CSCs to the plasma membrane by facilitating exit from the TGN and/or interaction of CSC secretory vesicles with the plasma membrane.