Identifying Lipid Metabolites Influenced by Oleic Acid Administration Using High-Performance Liquid Chromatography-Mass Spectrometry-Based Lipidomics
Identifying Lipid Metabolites Influenced by Oleic Acid Administration Using High-Performance Liquid Chromatography-Mass Spectrometry-Based Lipidomics
复制标题
使用基于高效液相色谱-质谱的脂质组学鉴定受油酸施用影响的脂质代谢物
DOI：
10.1021/acsomega.9b04402
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发表时间：
2020-05-26
期刊：
影响因子：
4.1
通讯作者：
Liu，Rong
中科院分区：
文献类型：
--
作者：
Xu，Chao;Song，Dan;Liu，Rong
油酸(OA)是最重要的单不饱和脂肪酸之一，对慢性肝病(CLD)具有保护作用，但其代谢机制尚不清楚。基于高效液相-质谱仪的脂质组学被用来鉴定和量化当肝细胞暴露于OA时内源性改变的脂质代谢物。已鉴定的脂类可划分为22种脂类，其中10种脂类受到OA处理的显著影响：溶血磷脂酰胆碱(LPC)、磷脂酰甘油(PG)、神经酰胺(Cer)、己糖神经酰胺(Hex1Cer)、二己糖神经酰胺(Hex2Cer)、胆固醇酯(ChE)和辅酶(Co)，而二甘油(DG)、甘油三酯(TG)和酰基卡尼汀(ACCA)增加。此外，VIP列表(VIP>；1.0和P<；0.05)显示，478种脂类与OA给药有显著差异，这些分子可能是与OA给药联合使用的潜在生物标志物。综上所述，我们的研究结果提供了一个新的视角，通过脂类组学研究内源性脂代谢产物水平的改变来理解OA给药的影响。
Oleic acid (OA), one of the most important monounsaturated fatty acids, possesses protective properties against chronic liver disease (CLD) development, but the underlying metabolic metabolism remains unknown. HPLC–MS-based lipidomics was utilized to identify and quantify the endogenously altered lipid metabolites when hepatocytes were exposed to OA administration. The identified lipids could be grouped into 22 lipid classes; of which, 10 classes were significantly influenced by the OA treatment: lysophosphatidylcholine (LPC), phosphatidylglycerol (PG), ceramides (Cer), hexosylceramides (Hex1Cer), dihexosylceramides (Hex2Cer), cholesterol ester (ChE), and coenzyme (Co) were decreased, while diglyceride (DG), triglyceride (TG), and acyl carnitine (AcCa) were increased. In addition, as the variable importance in projection (VIP) list (VIP > 1.0 andP< 0.05) showed, 478 lipid species showed significant difference with OA administration, and these molecules could be potential biomarkers in conjunction with OA administration. In summary, our results provided a novel perspective to understand the influences of OA administration by investigating endogenous altered levels of lipid metabolites via lipidomics.