Continuous and overlapping expression domains of odorant receptor genes in the olfactory epithelium determine the dorsal/ventral positioning of glomeruli in the olfactory bulb
Continuous and overlapping expression domains of odorant receptor genes in the olfactory epithelium determine the dorsal/ventral positioning of glomeruli in the olfactory bulb
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DOI：
10.1523/jneurosci.0324-05.2005
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发表时间：
2005-04-06
影响因子：
5.3
通讯作者：
Sakano, H
中科院分区：
文献类型：
--
作者：
Miyamichi, K;Serizawa, S;Sakano, H
在哺乳动物中，嗅觉上皮（OE）中的气味受体（OR）接收的嗅觉信号被转换为嗅球（OB）中激活的肾小球的地形图。据报道，OE可以分为四个地形不同的区域和嗅觉感觉神经元（OSNs）表达一个特定的OR基因随机分布在一个区域内。在这里，我们分析了80个不同的II类或基因的表达模式在OE原位杂交。发现OE中的表达区域并不总是适合四个常规区域之一。表达区域是特定于每个OR基因的，并且在OE中以重叠和连续的方式排列。我们还分析了OE和OB之间的空间关系用于OSN投影。我们的转基因以及DiI逆行染色实验表明，在OB的肾小球的背/腹侧排列与相应的OR的表达区域沿着的背内侧/腹外侧轴在OE。本研究表明，OR基因的选择可能会受到更多的限制OSN的位置在OE比什么已经被认为。
In mammals, olfactory signals received by odorant receptors (ORs) in the olfactory epithelium (OE) are converted to a topographical map of activated glomeruli in the olfactory bulb ( OB). It has been reported that the OE can be divided into four topographically distinct zones and that olfactory sensory neurons (OSNs) expressing a particular OR gene are randomly distributed within one zone. Here, we analyzed 80 different class II OR genes for their expression patterns in the OE by in situ hybridization. It was found that the expression area in the OE does not always fit into one of the four conventional zones. Expression areas are specific to each OR gene and are arranged in an overlapping and continuous manner in the OE. We also analyzed a spatial relationship between the OE and the OB for OSN projection. Our transgenic as well as DiI retrograde staining experiments demonstrated that the dorsal/ventral arrangement of glomeruli in the OB is correlated with the expression areas of corresponding ORs along the dorsomedial/ventrolateral axis in the OE. The present study indicates that the OR gene choice may be more restricted by the OSN location in the OE than what has been thought.