Rx3 and Shh direct anisotropic growth and specification in the zebrafish tuberal/anterior hypothalamus.
Rx3 and Shh direct anisotropic growth and specification in the zebrafish tuberal/anterior hypothalamus.
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DOI：
10.1242/dev.138305
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发表时间：
2016-07-15
期刊：
影响因子：
--
通讯作者：
Placzek M
中科院分区：
文献类型：
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作者：
Muthu V;Eachus H;Ellis P;Brown S;Placzek M
在发育中的大脑中，生长和分化密切相关。在这里，我们证明在斑马鱼胚胎中，同源域转录因子 Rx3 协调这些过程以构建结节/前下丘脑。 rx3 chk 突变体/rx3 morphant 鱼的分析和 EdU 脉冲追踪研究表明，rx3 是选择结节/前下丘脑祖细胞并协调其各向异性生长所必需的。在缺乏 Rx3 功能的情况下，祖细胞会在第三心室壁中积聚、死亡或被不当指定，shh+ 前隐窝不会形成，并且其驻留的 pomc+、ff1b+ 和 otpb+ Th1+ 细胞无法分化。对 Shh 信号传导的操纵表明，Shh 通过充当 rx3 的开关来协调祖细胞的选择和行为。我们的研究共同表明，Shh 和 Rx3 控制着独特祖细胞结构域的形成，该祖细胞结构域通过其各向异性生长和分化来阐述图案。摘要：在斑马鱼结节/下丘脑前部发育过程中，Shh 和 Rx3 控制着独特祖细胞结构域的形成，该祖细胞结构域通过其各向异性生长和分化来阐述模式。
In the developing brain, growth and differentiation are intimately linked. Here, we show that in the zebrafish embryo, the homeodomain transcription factor Rx3 coordinates these processes to build the tuberal/anterior hypothalamus. Analysis of rx3 chk mutant/rx3 morphant fish and EdU pulse-chase studies reveal that rx3 is required to select tuberal/anterior hypothalamic progenitors and to orchestrate their anisotropic growth. In the absence of Rx3 function, progenitors accumulate in the third ventricular wall, die or are inappropriately specified, the shh+ anterior recess does not form, and its resident pomc+, ff1b+ and otpb+ Th1+ cells fail to differentiate. Manipulation of Shh signalling shows that Shh coordinates progenitor cell selection and behaviour by acting as an on-off switch for rx3. Together, our studies show that Shh and Rx3 govern formation of a distinct progenitor domain that elaborates patterning through its anisotropic growth and differentiation. Summary: During zebrafish tuberal/anterior hypothalamus development, Shh and Rx3 govern the formation of a distinct progenitor domain that elaborates pattern through its anisotropic growth and differentiation.