Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice
Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice
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DOI：
10.3791/51413
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发表时间：
2014-04-01
影响因子：
1.2
通讯作者：
Kolber, Benedict J.
中科院分区：
文献类型：
--
作者：
Sadler, Katelyn E.;Stratton, Jarred M.;Kolber, Benedict J.
在美国，大约有3-8百万人患有间质性膀胱炎/膀胱疼痛综合征（IC/BPS），这是一种衰弱性病症，其特征在于排尿的紧迫性和频率增加，以及排尿困难和一般骨盆疼痛，尤其是在膀胱充盈或排尿时。尽管多年的研究，IC/BPS的原因仍然难以捉摸，治疗策略无法为患者提供完全缓解。为了研究神经系统对疾病的贡献，已经开发了许多动物模型来模拟与IC/BPS相关的疼痛和症状。一种这样的鼠模型是膀胱扩张（UBD）。在该模型中，特定压力的压缩空气在设定的时间段内输送到轻度麻醉动物的膀胱。在整个手术过程中，上级腹斜肌中的导线记录来自肌肉的电活动。这种活性被称为内脏反应（VMR），是一种可靠且可重复的伤害感受测量方法。在这里，我们描述了在小鼠中执行该技术所需的步骤，包括手术操作，生理记录和数据分析。通过使用该模型，可以解开初级感觉神经元、脊髓次级传入神经和涉及膀胱疼痛的高级中枢神经系统区域之间的协调。这些基本的科学知识可以在临床上转化为治疗IC/BPS患者。
Approximately 3-8 million people in the United States suffer from interstitial cystitis/bladder pain syndrome (IC/BPS), a debilitating condition characterized by increased urgency and frequency of urination, as well as nocturia and general pelvic pain, especially upon bladder filling or voiding. Despite years of research, the cause of IC/BPS remains elusive and treatment strategies are unable to provide complete relief to patients. In order to study nervous system contributions to the condition, many animal models have been developed to mimic the pain and symptoms associated with IC/BPS. One such murine model is urinary bladder distension (UBD). In this model, compressed air of a specific pressure is delivered to the bladder of a lightly anesthetized animal over a set period of time. Throughout the procedure, wires in the superior oblique abdominal muscles record electrical activity from the muscle. This activity is known as the visceromotor response (VMR) and is a reliable and reproducible measure of nociception. Here, we describe the steps necessary to perform this technique in mice including surgical manipulations, physiological recording, and data analysis. With the use of this model, the coordination between primary sensory neurons, spinal cord secondary afferents, and higher central nervous system areas involved in bladder pain can be unraveled. This basic science knowledge can then be clinically translated to treat patients suffering from IC/BPS.