Hexadecanoic Acid from Buzhong Yiqi Decoction Induced Proliferation of Bone Marrow Mesenchymal Stem Cells
Hexadecanoic Acid from Buzhong Yiqi Decoction Induced Proliferation of Bone Marrow Mesenchymal Stem Cells
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补中益气汤十六酸诱导骨髓间充质干细胞增殖
DOI：
10.1089/jmf.2009.1293
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发表时间：
2010-08-01
影响因子：
2.4
通讯作者：
Hua, Zi-Chun
中科院分区：
文献类型：
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作者：
Chen, Dong-Feng;Li, Xican;Hua, Zi-Chun
补中益气汤是一个著名的中医古方。本研究的目的是鉴定BYD中参与促进骨髓间充质干细胞（MSCs）增殖的活性成分，并探讨其作用机制。BYD用石油醚、乙醇和水提取。3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑、溴脱氧尿苷、增殖细胞核抗原免疫反应性、细胞周期分析和气相色谱-质谱分析提供的证据表明，用石油醚提取的BYD中的十六烷酸（HA）是负责增加MSC增殖的活性化合物。Western blot分析显示HA可显著增加MSCs的视黄酸受体（RAR）水平，但对雌激素受体、甲状腺激素受体、维生素D受体、糖皮质激素受体和过氧化物酶体增殖物激活受体无明显影响。逆转录聚合酶链反应显示HA显着增加RAR mRNA水平。此外，HA的作用机制依赖于RAR途径，并上调RAR靶基因胰岛素样生长因子-I的mRNA表达。我们的研究结果使我们对中医药在干细胞药理学调控方面的理解更加完善，并可能对干细胞生物学和治疗有用。
Buzhong Yiqi decoction (BYD) is a well-known ancient tonic prescription in traditional Chinese medicine (TCM). The purpose of this study is to identify active components of BYD involved in promoting proliferation of mesenchymal stem cells (MSCs) and to investigate its mechanism. BYD was extracted with petroleum ether, ethanol, and water. Evidence provided by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, bromodeoxyuridine, proliferation cell nuclear antigen immunoreactivity, cell cycle analysis, and gas chromatography-mass spectrometry indicated that hexadecanoic acid (HA) in BYD extracted with petroleum ether is the active compound responsible for increasing proliferation of MSCs. Western blot analysis show that HA significantly increase retinoic acid receptor (RAR) levels of MSCs, but not estrogen receptor, thyroid hormone receptor, vitamin D receptor, glucocorticoid receptor, and peroxisome proliferator-activated receptor. Reverse transcription-polymerase chain reaction revealed that HA significantly increased RAR mRNA levels. Furthermore, the mechanism of HA action depends on RAR pathway and up-regulates expression of mRNA for insulin-like growth factor-I, the target gene of RAR. Our findings have now allowed for a refinement in our understanding of TCM with respect to pharmacological regulation of stem cells and may be useful to stem cell biology and therapy.