The major late transcription factor binds to and activates the mouse metallothionein I promoter.
The major late transcription factor binds to and activates the mouse metallothionein I promoter.
复制标题
主要的晚期转录因子结合并激活小鼠金属硫蛋白 I 启动子。
DOI：
10.1101/gad.1.9.973
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发表时间：
1987
影响因子：
10.5
通讯作者：
Sharp,PA
中科院分区：
文献类型：
--
作者：
Carthew,RW;Chodosh,LA;Sharp,PA
人（HeLa）细胞含有蛋白质MLTF，其特异性结合腺病毒2主要晚期启动子中的DNA序列并激活该启动子的转录。未感染细胞中MLTF的存在表明该因子有助于某些细胞基因的转录。我们发现MLTF以序列特异性方式结合到小鼠金属硫蛋白I（mMTI）基因的5 '侧翼区。通过DNA结合凝胶电泳分析和DNA甲基化干扰分析，结合定位在-101和-94之间（相对于+1的起始位点）。与腺病毒一样，结合发生在含有序列CPuCGTGAC的区域。该序列的缺失既消除了MLTF的结合，又使体外转录效率降低了四倍。与完整启动子相反，从缺失突变启动子的转录没有通过向体外重建反应中加入纯化的MLTF来刺激。这些结果表明，MLTF有助于细胞基因的转录。
Human (HeLa) cells contain a protein, MLTF, which specifically binds to a DNA sequence in the adenovirus 2 major late promoter and activates transcription of that promoter. The presence of MLTF in uninfected cells suggests that this factor contributes to the transcription of some cellular genes. We find that MLTF binds in a sequence-specific manner to the 5'-flanking region of the mouse metallothionein I (mMTI) gene. Binding was localized between -101 and -94 (relative to the initiation site at +1) by DNA-binding gel electrophoresis assay and DNA methylation interference analysis. As in adenovirus, binding occurred in a region containing the sequence CPuCGTGAC. Deletion of this sequence both eliminated the binding of MLTF and produced a fourfold reduction in transcriptional efficiency in vitro. In contrast to the intact promoter, transcription from the deletion mutant promoter was not stimulated by addition of purified MLTF to an in vitro reconstituted reaction. These results suggest that MLTF contributes to the transcription of cellular genes.