Static mechanical behavior of bronchi in excised dog lung.
Static mechanical behavior of bronchi in excised dog lung.
复制标题
离体狗肺中支气管的静态机械行为。
DOI：
10.1152/jappl.1974.37.2.201
复制
发表时间：
1974
影响因子：
3.3
通讯作者：
R. Hyatt
中科院分区：
文献类型：
--
作者：
R. Sittipong;R. Hyatt
方法取杂种犬15只，雌雄各半。体重平均为17 kg，范围为8 - 50 kg。通过静脉注射戊巴比妥钠处死犬，并立即取出肺。允许血液从肺血管中排出，不结扎肺血管。将左上叶（LUL）与肺的其余部分分离，并将套管系在其支气管中。切除肺叶的舌部，使肺叶不透气。选择LUL进行研究，因为它是平坦的，并且因为在去除小舌的情况下，它的边缘几乎没有卷曲或旋转。我们认为，出于几个原因，切除小舌并没有干扰我们的测量。首先，切除的小舌残端总是远离任何选择用于测量的支气管。这在图1中是明显的，其中舌残端邻近套管的端部。此外，已经表明，胸膜表面的改变，例如由我们的小舌切除手术产生的改变，仅引起肺泡几何形状的局部变形，其紧密地限制于胸膜表面（24），因此不太可能影响肺叶的整体弹性行为。用水性造影剂勾画LUL的气道。注意使用尽可能少的造影剂，以避免阻塞小气道。将约2 mm长的金属标记物粘在肺叶边缘（图1）。将LUL水平放置在潮湿的玻璃板上，X射线胶片放置在玻璃板下。初步研究表明，在水平位置时，充气和放气在更宽的体积范围内比叶垂直悬挂时更均匀。
METHODSExcised lungs from 15 mongrel dogs of both sexes were studied. Body weight averaged 17 kg, with a range from 8 to 50 kg. The dogs were sacrificed by the use of intravenously administered sodium pentobarbital, and the lungs were removed immediately. Blood was allowed to drain from the pulmonary vessels, which were not ligated. The left upper lobe (LUL) was separated from the rest of the lung, and a cannula was tied into its bronchus. The lingular portion of the lobe was removed and the lobe rendered airtight. The LUL was selected for study because it is flat and because, with the lingula removed, it expands with little curling or rotation of its edges. We believe that removal of the lingula did not interfere with our measurements for several reasons. First, the amputated stump of the lingula was always far removed from any bronchi selected for measurement. This is evident in Fig. 1, where the lingular stump is adjacent to the end of the cannula. Furthermore, it has been shown that alterations in the pleural surface, such as were produced by our procedure of lingula removal, cause only local distortions in alveolar geometry which are closely limited to the pleural surface (24) and hence are unlikely to affect the overall elastic behavior of the lobe. The airways of the LUL were outlined with aqueous contrast material. Care was taken to use as little contrast medium as possible to avoid occluding the small airways. Metal markers, approximately 2 mm long, were glued to the lobe edges (Fig. 1). The LUL was placed horizontally on a moistened glass plate, under which the X-ray film was posi-tioned. Preliminary studies had shown that inflation and deflation were more uniform over a wider volume range in the horizontal position than when the lobe was suspended vertically.