Distinct Mineral Weathering Behaviors of the Novel Mineral-Weathering Strains Rhizobium yantingense H66 and Rhizobium etli CFN42
Distinct Mineral Weathering Behaviors of the Novel Mineral-Weathering Strains Rhizobium yantingense H66 and Rhizobium etli CFN42
复制标题
新型矿物风化菌株Rhizobium yantingense H66和Rhizobium etli CFN42的独特矿物风化行为
DOI：
10.1128/aem.00918-16
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发表时间：
2016-07-01
影响因子：
4.4
通讯作者：
Sheng, Xia-Fang
中科院分区：
文献类型：
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作者：
Chen, Wei;Luo, Long;Sheng, Xia-Fang
细菌在矿物风化、土壤形成和元素循环中发挥着重要作用。然而，人们对硅酸盐矿物和根瘤菌之间的相互作用知之甚少。本研究比较了Rhizobium yantingense H66（一种新型矿物风化根瘤菌）和Rhizobium etli CFN42在矿物风化过程中的钾长石风化、矿物表面吸附和代谢活性。与菌株 CFN42 相比，菌株 H66 显示出明显更高的从矿物中动员的 Si、Al 和 K，以及矿物表面上的细胞数与总细胞数的比率更高。虽然这两个菌株产生葡萄糖酸，但菌株 H66 在低葡萄糖和高葡萄糖介质中的矿物风化过程中也产生乙酸、苹果酸和琥珀酸。值得注意的是，与高葡萄糖培养基相比，低葡萄糖培养基中菌株 H66 的 Si、Al 和 K 释放量更高，矿物表面细胞数与总细胞数的比率更高，并且有机酸产量更高。矿物表面的扫描电子显微镜分析和冗余分析表明，矿物表面细胞吸附和矿物风化之间存在更强的正相关性，这由溶解的铝和钾浓度表明。结果表明，两种根瘤菌在矿物风化方面表现不同。结果表明，盐亭根瘤菌 H66 通过增加细胞对矿物表面的吸附以及低营养浓度和高营养浓度下葡萄糖代谢的差异，特别是在低营养浓度下促进钾长石风化。 重要性本研究报道了钾长石风化、矿物表面的细胞吸附能力，以及新型矿物风化根瘤菌盐廷根 H66 和根瘤菌 etli CFN42 在不同营养条件下的代谢差异。条件。结果表明，盐亭根瘤菌H66在低糖和高糖培养基中均具有较强的矿物风化能力，尤其是在低糖培养基中。此外，盐亭根瘤菌 H66 通过增加细胞对矿物表面的吸附和增加有机酸的产生来促进矿物风化。我们的研究结果使我们能够更好地理解不同根瘤菌在各种土壤环境中的硅酸盐矿物风化、元素循环和土壤形成中的作用，从而更深入地了解根瘤菌对这些过程的地球微生物贡献。
Bacteria play important roles in mineral weathering, soil formation, and element cycling. However, little is known about the interaction between silicate minerals and rhizobia. In this study, Rhizobium yantingense H66 (a novel mineral-weathering rhizobium) and Rhizobium etli CFN42 were compared with respect to potash feldspar weathering, mineral surface adsorption, and metabolic activity during the mineral weathering process. Strain H66 showed significantly higher Si, Al, and K mobilization from the mineral and higher ratios of cell numbers on the mineral surface to total cell numbers than strain CFN42. Although the two strains produced gluconic acid, strain H66 also produced acetic, malic, and succinic acids during mineral weathering in low- and high-glucose media. Notably, higher Si, Al, and K releases, higher ratios of cell numbers on the mineral surface to total cell numbers, and a higher production of organic acids by strain H66 were observed in the low-glucose medium than in the high-glucose medium. Scanning electron microscope analyses of the mineral surfaces and redundancy analysis showed stronger positive correlations between the mineral surface cell adsorption and mineral weathering, indicated by the dissolved Al and K concentrations. The results showed that the two rhizobia behaved differently with respect to mineral weathering. The results suggested that Rhizobium yantingense H66 promoted potash feldspar weathering through increased adsorption of cells to the mineral surface and through differences in glucose metabolism at low and high nutrient concentrations, especially at low nutrient concentrations.IMPORTANCEThis study reported the potash feldspar weathering, the cell adsorption capacity of the mineral surfaces, and the metabolic differences between the novel mineral-weathering Rhizobium yantingense H66 and Rhizobium etli CFN42 under different nutritional conditions. The results showed that Rhizobium yantingense H66 had a greater ability to weather the mineral in low-and high-glucose media, especially in the low-glucose medium. Furthermore, Rhizobium yantingense H66 promoted mineral weathering through the increased adsorption of cells to the mineral surface and through increased organic acid production. Our results allow us to better comprehend the roles of different rhizobia in silicate mineral weathering, element cycling, and soil formation in various soil environments, providing more insight into the geomicrobial contributions of rhizobia to these processes.