Biochemical activities of N,O-carboxymethyl chitosan from squid cartilage
Biochemical activities of N,O-carboxymethyl chitosan from squid cartilage
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DOI：
10.1016/j.carbpol.2011.04.007
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发表时间：
2011-07
影响因子：
11.2
通讯作者：
Dongke Zhao;Jun Huang;Sheng Hu;Jian-wei Mao;L. Mei
中科院分区：
文献类型：
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作者：
Dongke Zhao;Jun Huang;Sheng Hu;Jian-wei Mao;L. Mei
以鱿鱼软骨β-甲壳素为原料，经碱脱乙酰化制备壳聚糖，并合成了不同取代度的羧甲基化衍生物。通过pH滴定法计算的衍生物的DS分别为0.64、0.81、1.0、1.33和1.59。研究了衍生物的体外抗氧化性能和胆汁酸结合能力。壳聚糖的羧甲基化使其与胆汁酸的结合能力增强。在1 mg/mL浓度下，羧甲基壳聚糖（CMCS）对DPPH自由基的清除作用强于壳聚糖。羧甲基壳聚糖对超氧阴离子自由基的清除作用强于壳聚糖，EC 50值小于5.6mg/mL。降低功耗的效果与CMCS的DS相关。当浓度为1.2 mg/mL时，CMCS对二价铁的螯合能力为100%，而壳聚糖的螯合能力仅为22.34%。这表明羧甲基化是获得具有良好生物学性能的壳聚糖衍生物的一种可能途径。
Chitosan was prepared by alkalineN-deacetylation of squid cartilage β-chitin and carboxymethylated derivatives with different degrees of substitution (DS) were synthesized. The DS of the derivatives calculated by pH titration were 0.64, 0.81, 1.0, 1.33 and 1.59. The antioxidant properties and bile acid binding capacity of the derivatives were studiedin vitro. The carboxymethylation of chitosan caused enhancement of bile acid binding capacity. At 1 mg/mL, carboxymethyl chitosan (CMCS) showed a stronger scavenging effect than chitosan towards DPPH radicals. The CMCS scavenging effect on superoxide radicals was stronger than that of chitosan, and EC50values were below 5.6 mg/mL. The effectiveness of reducing power correlated with the DS of CMCS. At 1.2 mg/mL, the ability of CMCS to chelate ferrous iron was 100%, whereas that of chitosan was only 22.34%. This suggested that carboxymethylation is a possible approach to obtain chitosan derivatives with desirable biological properties.