Canine cyclic haematopoiesis: bone marrow adherent cell influence of CFU-C formation.
Canine cyclic haematopoiesis: bone marrow adherent cell influence of CFU-C formation.
复制标题
犬循环造血：骨髓贴壁细胞对 CFU-C 形成的影响。
DOI：
10.1111/j.1365-2141.1982.tb01961.x
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发表时间：
1982
影响因子：
6.5
通讯作者：
Jolly,JD
中科院分区：
文献类型：
--
作者：
Jones,JB;Jolly,JD
犬循环造血（CH）似乎是一种多能干细胞缺陷，可能是由于内在的骨髓缺陷所致。将体外循环造血（CH）狗的贴壁骨髓细胞培养为正常狗非贴壁骨髓细胞的下层。正常狗非贴壁细胞的骨髓粒细胞定型集落形成单位 (CFU-C) 受到 CH 贴壁细胞底层的周期性影响。中性粒细胞减少症发作后第九天或第十天收集的 CH 贴壁细胞不会刺激 CFU-C 形成，而第六天收集的细胞则刺激多达 108 个集落。在其他周期日收集的 CH 贴壁细胞支持 CFU-C 形成增加，但周期第 3 天除外，该细胞不一致地刺激生长。这些数据表明CH骨髓体外贴壁细胞交替刺激和抑制正常狗骨髓的体外粒细胞生成。
Canine cyclic haematopoiesis (CH) appears to be a multipotential stem cell defect, possibly due to an intrinsic marrow defect. Thein vitroadherent marrow cells of the cyclic haematopoietic (CH) dog were cultured as underlayers beneath normal dog nonadherent marrow cells. The marrow granulocyte‐committed colony forming units (CFU‐C) of the normal dog nonadherent cells were cyclically influenced by the CH adherent cell underlayers. The CH adherent cells collected on the ninth or tenth day following the onset of neutropenia did not stimulate CFU‐C formation while those collected on the sixth day stimulated as many as 108 colonies. The CH adherent cells collected on other cycle days supported increased CFU‐C formation with the exception of cycle day 3 which inconsistently stimulated growth. These data show that the CH marrowin vitroadherent cells alternately stimulate and inhibitin vitrogranulopoiesis of normal dog marrows.