The clinical significance of Aurora-A/STK15/BTAK expression in human esophageal squamous cell carcinoma
The clinical significance of Aurora-A/STK15/BTAK expression in human esophageal squamous cell carcinoma
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DOI：
10.1158/1078-0432.ccr-04-1627
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发表时间：
2005-03-01
影响因子：
11.5
通讯作者：
Shimada, Y
中科院分区：
文献类型：
--
作者：
Tanaka, E;Hashimoto, Y;Shimada, Y
目的：Aurora-A/STK15/BTAK(Aurora-A)编码一种与染色体分布相关的丝氨酸/苏氨酸激酶，其上调导致染色体不稳定，从而导致多种类型癌症的非整倍体和细胞转化。本研究旨在探讨Aurora-A在人食管鳞癌中的作用。实验设计：采用半定量逆转录-聚合酶链式反应方法，比较33例食管鳞癌组织和相应正常组织中Aurora-A基因的表达水平，并用免疫组织化学方法检测142例食管鳞癌组织中Aurora-A蛋白的分布和表达水平。结果：半定量逆转录聚合酶链式反应检测30%(10/33)的食管鳞癌组织中Aurora-A基因表达上调，免疫组化检测53%(75/142)的食管鳞癌组织中Aurora-A蛋白表达上调。Aurora-A基因和蛋白表达上调与远处淋巴结转移相关(P=0.003和P=0.04)，且Aurora-A基因和蛋白表达上调患者预后较差(P=0.003和P=0.0009)。此外，多因素分析显示Aurora-A蛋白上调是一个独立的预后因素。结论：Aurora-A的表达上调可能反映了ESCC的恶性行为，可作为判断ESCC患者预后的有用指标。
Purpose: Aurora-A/STK15/BTAK (Aurora-A) encodes a Serine/Threonine kinase associated with chromosomal distribution, and its up-regulation induces chromosomal instability thereby leading to aneuploidy and cell transformation in several types of cancer. In this study, we investigated the role of Aurora-A in human esophageal squamous cell carcinoma (ESCC).Experimental Design: The expression levels of Aurora-A mRNA were compared in 33 ESCC tissues with that in corresponding normal esophageal epithelium by semiquantitative reverse transcription-PCR, and the distribution patterns and expression levels of Aurora-A protein were immunohistochemically investigated in the ESCC tumors of 142 patients. The results were then separately compared with the clinicopathologic findings of the patients, and the expression of Aurora-A was examined in nine ESCC cell lines and a normal esophageal epithelial cell line using Western blot analysis.Results: The up-regulation of Aurora-A mRNA was found in 30% (10 of 33) of the tumors by semiquantitative reverse transcription-PCR, and protein up-regulation was found in 53% (75 of 142) of the patients by immunohistochemistry. mRNA and protein up-regulation of Aurora-A were correlated with distant lymph node metastasis (P = 0.05 and P = 0.04, respectively), and patients with Aurora-A mRNA or protein up-regulation had a poorer prognosis (P = 0.003 and P = 0.0009, respectively). Furthermore, multivariate analysis revealed that up-regulation of the Aurora-A protein was an independent prognostic factor. In addition, Aurora-A expression in all ESCC cell lines was higher than that in a normal esophageal epithelial cell line.Conclusions: The up-regulation of Aurora-A expression may reflect the malignant behavior of ESCC and may prove useful information as a prognostic factor for ESCC patients.