Biocontrol and Rapid Detection of Food-Borne Pathogens Using Bacteriophages and Endolysins.
Biocontrol and Rapid Detection of Food-Borne Pathogens Using Bacteriophages and Endolysins.
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DOI：
10.3389/fmicb.2016.00474
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发表时间：
2016
影响因子：
5.2
通讯作者：
Lee JH
中科院分区：
文献类型：
--
作者：
Bai J;Kim YT;Ryu S;Lee JH
噬菌体是天然的食品防腐剂，也是快速检测食品中食源性致病菌的材料。自从单核细胞增生李斯特菌靶向噬菌体混合物（ListShield）被批准用于食品中，许多噬菌体已被筛选和实验表征用于食品中的噬菌体应用。采用单一噬菌体和噬菌体混合物对大肠杆菌等食源性致病菌污染的食品进行了处理。大肠杆菌O157：H7、肠道沙门氏菌、空肠弯曲杆菌、单核细胞增生李斯特菌、金黄色葡萄球菌、坂崎克罗伊氏菌和弧菌。结果表明，它们对控制各种食源性病原体具有很大的潜力，并可能成为传统食品防腐剂的替代品。此外，具有高宿主特异性和宿主裂解活性的噬菌体来源的内溶素可能优选用于食品应用而不是微生物。为了快速检测食源性病原体，由于内溶素的细胞壁结合结构域（CBD）具有高宿主特异性结合，因此建议使用它们。生物标记的CBD已被成功地评估，并建议作为昂贵的抗体的替代材料用于各种检测应用。最近，已经开发和测试了报告噬菌体系统，以确认其用于特异性检测的可用性和准确性。这些系统显示了一些优点，如在很短的反应时间内仅快速检测活的病原细胞而不受食品成分的干扰，这表明这些系统可能适合于监测食品中的病原体。因此，噬菌体是下一代生物防治剂，也是确认甚至鉴定各种食品中存在的食源性病原体的快速检测工具。
Bacteriophages have been suggested as natural food preservatives as well as rapid detection materials for food-borne pathogens in various foods. Since Listeria monocytogenes-targeting phage cocktail (ListShield) was approved for applications in foods, numerous phages have been screened and experimentally characterized for phage applications in foods. A single phage and phage cocktail treatments to various foods contaminated with food-borne pathogens including E. coli O157:H7, Salmonella enterica, Campylobacter jejuni, Listeria monocytogenes, Staphylococcus aureus, Cronobacter sakazakii, and Vibrio spp. revealed that they have great potential to control various food-borne pathogens and may be alternative for conventional food preservatives. In addition, phage-derived endolysins with high host specificity and host lysis activities may be preferred to food applications rather than phages. For rapid detection of food-borne pathogens, cell-wall binding domains (CBDs) from endolysins have been suggested due to their high host-specific binding. Fluorescence-tagged CBDs have been successfully evaluated and suggested to be alternative materials of expensive antibodies for various detection applications. Most recently, reporter phage systems have been developed and tested to confirm their usability and accuracy for specific detection. These systems revealed some advantages like rapid detection of only viable pathogenic cells without interference by food components in a very short reaction time, suggesting that these systems may be suitable for monitoring of pathogens in foods. Consequently, phage is the next-generation biocontrol agent as well as rapid detection tool to confirm and even identify the food-borne pathogens present in various foods.