Immunohistochemical expression of Ki-67 antigen, Cox-2 and Bax/Bcl-2 in prostate cancer;: Prognostic value in biopsies and radical prostatectomy specimens
Immunohistochemical expression of Ki-67 antigen, Cox-2 and Bax/Bcl-2 in prostate cancer;: Prognostic value in biopsies and radical prostatectomy specimens
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DOI：
10.1016/j.eururo.2005.06.014
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发表时间：
2005-11-01
期刊：
影响因子：
23.4
通讯作者：
Llombart-Bosch, A
中科院分区：
文献类型：
--
作者：
Rubio, J;Ramos, D;Llombart-Bosch, A
目的：探讨前列腺癌(PC)活检标本(C)和手术标本(SP)中Bcl2、Bax、COX-2和Ki-67抗原的免疫组织化学(IHC)表达对前列腺癌(PC)预后的预测价值，并确定C到SP的可重复性。材料和方法：对91例前列腺癌根治术患者进行了IHC研究。Bax在几乎所有患者中都有表达，且无任何预后价值。我们观察到除Bax以外的所有分子在C和SP之间具有良好的重现性。在前列腺癌中，Ki-67和COX-2的阳性表达率分别为42.9%和67%，单因素分析显示Ki-67和COX-2与前列腺癌的无瘤生存期相关。这两种标记物在SP中的表达分别为51.6%和79.1%，在与无病生存相关的多因素分析中，Ki-67在SP中的表达保持了其作为预后因素的独立性。结论：本研究中Ki-67和COX-2蛋白的IHC表达确实提供了有价值的预后信息，主要是第一种。因此，我们认为这些标记物可能会在更大范围的患者中进行研究，以进一步验证其作为前列腺活检预后因素的有效性。(C)2005 Elsevier B.V.保留所有权利。
Purposes: To elucidate the prognostic value of the immunohistochemical (IHC) expression of Bcl-2, Bax, Cox-2 and Ki-67 antigen in biopsy cores (C) and surgical specimens (SP) of prostate cancer (PC) and to determine the C to SP reproducibility.Material and methods: The IHC study was carried out in 91 patients operated by means of radical prostatectomy (RP) with available formalin-fixed paraffin-embedded material from both C and SP.Results: The IHC expression of Bcl-2 in C and SP was very low (5%). Bax was expressed in almost all the patients and did not show any prognostic value. We observed a good reproducibility between C and SP for all molecules except with Bax. In prostate C, Ki-67 and Cox-2 were considered positive in 42.9% and 67% of the patients respectively, and were related to disease-free survival in the univariate analysis. The expression of these two markers in SP was observed in 51.6% and 79.1% of the patients and the expression of Ki-67 in SP maintained its independence as prognostic factor in the multivariate analysis related to disease-free survival.Conclusions: The IHC expression of Ki-67 and Cox-2 proteins in our study do offer valuable prognostic information, mostly the first one. Thus, we think these markers might be studied in larger series of patients for its further validation as prognostic factors in prostate biopsies. (c) 2005 Elsevier B.V. All rights reserved.