Virulence of six capsular serotypes of Porphyromonas gingivalis in a mouse model
Virulence of six capsular serotypes of Porphyromonas gingivalis in a mouse model
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DOI：
10.1111/j.1399-302x.1998.tb00714.x
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发表时间：
1998-10-01
影响因子：
--
通讯作者：
van Winkelhoff, AJ
中科院分区：
文献类型：
--
作者：
Laine, ML;van Winkelhoff, AJ
牙龈卟啉单胞菌的荚膜结构与动物模型中的致病性相关。最近在牙龈卟啉单胞菌中描述了六种多糖荚膜血清型。在本研究中，在小鼠模型中比较了六种荚膜血清型的牙龈卟啉单胞菌菌株与牙龈卟啉单胞菌的非荚膜血清型菌株的毒力。所有18种包囊菌株均引起渗出物扩散型感染，通常伴有皮肤溃疡和坏死以及体重显著减轻。所有三个非封装测试菌株诱导局部感染，虽然偶尔观察到传播型感染。小鼠的疾病几乎总是与从血液、肾脏、肺和脾脏中回收封装的牙龈卟啉单胞菌有关。在实验期间，在感染K-血清型菌株的小鼠和感染包封的牙龈卟啉单胞菌菌株的小鼠之间的平均体重变化中发现统计学上显著的对比。记录了相同血清型菌株之间疾病严重程度和从小鼠组织中恢复牙龈卟啉单胞菌的差异。在荚膜血清型内的毒力差异表明荚膜是牙龈卟啉单胞菌的重要但不是唯一的决定性毒力因子。
Capsular structures of Porphyromonas gingivalis have been correlated to the pathogenicity in animal models. Six polysaccharide capsular serotypes have recently been described in P. gingivalis. In the present study, virulence of the P. gingivalis strains of the six capsular serotypes was compared with strains of the non-capsular serotype of P. gingivalis in a mouse model. All 18 encapsulated strains caused a spreading type of infection with exudate, often accompanied by ulceration and necrosis of the skin and a significant loss of body weight. All three non-encapsulated strains tested induced localized abscesses, although a spreading type of infection was occasionally observed. Illness of the mice was almost always associated with recovery of encapsulated P. gingivalis from blood, kidneys, lungs and spleens. A statistically significant contrast was found in the average weight change during the experiment between the mice infected with the K- serotype strains and the mice infected with the encapsulated P. gingivalis strains. Differences in severity of illness and the recovery of P. gingivalis from mouse tissues were recorded between strains of the same serotype. The differences in virulence within a capsular serotype suggest that the capsule is an important but not the only determining virulence factor for P. gingivalis.