A Soybean bZIP Transcription Factor GmbZIP19 Confers Multiple Biotic and Abiotic Stress Responses in Plant
A Soybean bZIP Transcription Factor GmbZIP19 Confers Multiple Biotic and Abiotic Stress Responses in Plant
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DOI：
10.3390/ijms21134701
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发表时间：
2020-07-01
影响因子：
5.6
通讯作者：
Qin, Yuan
中科院分区：
文献类型：
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作者：
He, Qing;Cai, Hanyang;Qin, Yuan
碱性亮氨酸拉链(BZIP)是一个植物特有的转录因子家族，在响应生物和非生物胁迫中起着至关重要的作用。然而，目前对bZIP基因在大豆中的功能知之甚少。在本研究中，我们从大豆中分离到一个bZIP基因GmbZIP19。GmbZIP19的亚细胞定位研究揭示了其核定位。结果表明，脱落酸(ABA)、茉莉酸(JA)和水杨酸(SA)显著诱导GmbZIP19的表达，但在盐和干旱胁迫条件下GmbZIP19的表达降低。此外，GmbZIP19过表达拟南芥品系表现出更强的抗性。菌核菌和紫丁香假单胞菌与ABA、JA、ETH和SA诱导的标记基因表达上调有关，但对盐胁迫和干旱胁迫敏感，与气孔关闭破坏相关，并下调盐和干旱胁迫标记基因的表达。我们构建了一个大豆瞬时GmbZIP19过表达系，进行了染色质免疫沉淀分析，发现GmbZIP19与大豆ABA、JA、ETH和SA诱导的标记基因的启动子结合。酵母单杂交验证了这一组合。本研究表明，GmbZIP19是病原菌抗性的正调控因子，是耐盐性和抗旱性的负调控因子。
The basic leucine zipper (bZIP) is a plant-specific transcription factor family that plays crucial roles in response to biotic and abiotic stresses. However, little is known about the function of bZIP genes in soybean. In this study, we isolated a bZIP gene,GmbZIP19, from soybean. A subcellular localization study ofGmbZIP19revealed its nucleus localization. We showed thatGmbZIP19expression was significantly induced by ABA (abscisic acid), JA (jasmonic acid) and SA (salicylic acid), but reduced under salt and drought stress conditions. Further,GmbZIP19overexpressionArabidopsislines showed increased resistance toS. sclerotiorumandPseudomonas syringaeassociated with upregulated ABA-, JA-, ETH- (ethephon-)and SA-induced marker genes expression, but exhibited sensitivity to salt and drought stresses in association with destroyed stomatal closure and downregulated the salt and drought stresses marker genes' expression. We generated a soybean transientGmbZIP19overexpression line, performed a Chromatin immunoprecipitation assay and found that GmbZIP19 bound to promoters of ABA-, JA-, ETH-, and SA-induced marker genes in soybean. The yeast one-hybrid verified the combination. The current study suggested thatGmbZIP19is a positive regulator of pathogen resistance and a negative regulator of salt and drought stress tolerance.