Isolation and characterization of a cDNA clone specific for avian vitellogenin II.
Isolation and characterization of a cDNA clone specific for avian vitellogenin II.
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禽卵黄原蛋白 II 特异性 cDNA 克隆的分离和表征。
DOI：
10.1093/nar/10.16.4935
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发表时间：
1982
影响因子：
14.9
通讯作者：
Williams,DL
中科院分区：
文献类型：
--
作者：
Protter,AA;Wang,SY;Shelness,GS;Ostapchuk,P;Williams,DL
从雌激素诱导的公鸡肝脏cDNA文库中克隆了卵黄蛋白原（vitellogenin）基因。两种形式的血浆卵黄蛋白原，卵黄蛋白原I（VTG I）和卵黄蛋白原II（VTG II），区分其独特的部分蛋白水解图谱的基础上，已被表征和其相应的肝前体形式确定。我们已经使用这个标准来具体表征已克隆的卵黄蛋白原蛋白。VTG I和VTG II的标准，在体内合成的部分蛋白水解图进行了比较，在体外使用RNA杂交选择的卵黄蛋白质质粒合成的蛋白质的地图。八个主要的消化片段被发现共同的体外合成的卵黄蛋白原和VTG II标准，而没有观察到片段对应于VTG I地图。VTG II cDNA克隆的限制性图谱允许与先前描述的cDNA和基因组卵黄蛋白原克隆进行比较。
A clone for vitellogenin, a major avian, estrogen responsive egg yolk protein, was isolated from the cDNA library of estrogen-induced rooster liver. Two forms of plasma vitellogenin, vitellogenin I (VTG I) and vitelliogenin II (VTG II), distinguishable on the basis of their unique partial proteolysis maps, have been characterized and their corresponding hepatic precursor forms identified. We have used this criterion to specifically characterize which vitellogenin protein had been cloned. Partial proteolysis maps of VTG I and VTG II standards, synthesized in vivo, were compared to maps of protein synthesized in vitro using RNA hybrid-selected by the vitellogenin plasmid. Eight major digest fragments were found common to the in vitro synthesized vitellogenin and the VTG II standard while no fragments were observed to correspond to the VTG I map. A restriction map of the VTG II cDNA clone permits comparison to previously described cDNA and genomic vitellogenin clones.