RETINAL GANGLION-CELLS LOSE RESPONSE TO LAMININ WITH MATURATION
RETINAL GANGLION-CELLS LOSE RESPONSE TO LAMININ WITH MATURATION
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DOI：
10.1038/322465a0
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发表时间：
1986-07-31
期刊：
影响因子：
64.8
通讯作者：
BARTLETT, P
中科院分区：
文献类型：
--
作者：
COHEN, J;BURNE, JF;BARTLETT, P
神经元突起和培养底物之间的粘附性相互作用在决定轴突生长的形式和程度方面起着决定性的作用1，2已经极大地影响了关于脊椎动物神经系统中轴突生长和引导机制的想法。这些研究还有助于确定可能参与指导体内轴突生长的黏附分子。一个候选分子是层粘连蛋白，它是基底层的一种主要糖蛋白3，已被证明在培养4-8中能诱导多种胚胎神经元延伸轴突。此外，层粘连蛋白在能够成功再生的受损神经中大量存在，但在再生失败的神经中缺失。然而，当轴突生长开始时，调节轴突再生的机制在多大程度上也在胚胎中发挥作用尚不清楚。在这里，我们研究了由鸡胚胎视网膜神经节细胞体外培养出的轴突对底物的要求，视网膜中唯一的细胞将轴突发送到大脑。我们发现，虽然来自胚胎第6天(E6)雏鸡的视网膜神经节细胞在层粘连蛋白上延伸大量的轴突，但来自E11的那些细胞不会，尽管它们保留了通过层粘连蛋白不依赖的机制在星形胶质细胞上延伸轴突的能力。这是第一个证据表明，随着中枢神经系统神经元的成熟，其对突起生长的底物要求可能会发生变化。
The decisive role played by adhesive interactions between neuronal processes and the culture substrate in determining the form and extent of neurite outgrowthin vitro1,2has greatly influenced ideas about the mechanisms of axonal growth and guidance in the vertebrate nervous system. These studies have also helped to identify adhesive molecules that might be involved in guiding axonal growthin vivo. One candidate molecule is laminin, a major gly-coprotein of basal laminae3which has been shown to induce a wide variety of embryonic neurones to extend neurites in culture4–8. Moreover, laminin is found in large amounts in injured nerves that can successfully regenerate but is absent from nerves where regeneration fails9–11. However, it is unclear to what extent the mechanisms that regulate axonal regeneration also operate in the embryo when axon outgrowth is initiated. Here we have examined the substrate requirements for neurite outgrowthin vitroby chick embryo retinal ganglion cells, the only cells in the retina to send axons to the brain. We show that while retinal ganglion cells from embryonic day 6 (E6) chicks extend profuse neurites on laminin, those from Ell do not, although they retain the ability to extend neurites on astrocytes via a laminin-independent mechanism. This represents the first evidence that central nervous system neurones may undergo a change in their substrate requirements for neurite outgrowth as they mature.