Bifidobacterium infantis suppresses proinflammatory interleukin-17 production in murine splenocytes and dextran sodium sulfate-induced intestinal inflammation
Bifidobacterium infantis suppresses proinflammatory interleukin-17 production in murine splenocytes and dextran sodium sulfate-induced intestinal inflammation
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DOI：
10.3892/ijmm_00000006
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发表时间：
2008-08-01
影响因子：
5.4
通讯作者：
Saito, Yasuo
中科院分区：
文献类型：
--
作者：
Tanabe, Soichi;Kinuta, Yuki;Saito, Yasuo
白细胞介素（IL）-17作为一种有效的炎症细胞因子，产生IL-17的细胞（Th 17细胞）受到广泛关注。然而，还没有评估益生菌和/或益生菌在IL-17产生中的参与。在这项研究中，我们研究了五种细菌在体外和离体条件下对IL-17产生的抑制作用。在所研究的五种菌种中，双歧杆菌抑制IL-17的产生，但在TGF-β加IL-6刺激的小鼠脾细胞中最有效地增强IL-27的产生。B。葡聚糖硫酸钠处理的结肠器官培养物中的IL-17和嗜酸性粒细胞趋化因子的产生也受到抑制。B.在脾细胞和结肠培养物中均观察到了Escherichia coli，并认为其在一定程度上对抑制IL-17的产生是重要的。这些研究结果表明，一种新的免疫调节功能的肠道双歧杆菌，并进一步暗示，这些细菌可能是有用的治疗Th 17介导的疾病。
Interleukin (IL)-17 acts as a potent inflammatory cytokine, and IL-17-producing cells (Th17 cells) have received much attention. However, the involvement of commensal and/or probiotic bacteria in IL-17 production has not been evaluated. In this study, we examined the suppressive effects of five bacteria species on IL-17 production in vitro and ex vivo. Among the five species studied, Bifidobacterium infantis inhibited IL-17 production but enhanced IL-27 production most potently in TGF-beta plus IL-6-stimulated murine splenocytes. B. infantis also inhibited IL-17 and eotaxin production from a dextran sodium sulfate-treated colon organ culture. The induction of IL-10 by B. infantis was observed both in the splenocytes and in the colon culture and was assumed, to a certain extent, to be important for suppressing IL-17 production. These findings suggest a novel immunomodulatory function of commensal bifidobacteria and further imply that these bacteria may be useful in the treatment of Th17-mediated diseases.