Epigallocatechin-3-gallate increases autophagy signaling in resting and unloaded plantaris muscles but selectively suppresses autophagy protein abundance in reloaded muscles of aged rats.
Epigallocatechin-3-gallate increases autophagy signaling in resting and unloaded plantaris muscles but selectively suppresses autophagy protein abundance in reloaded muscles of aged rats.
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表没食子儿茶素-3-没食子酸酯可增加静息和卸载的跖肌中的自噬信号传导，但选择性抑制老年大鼠重载肌肉中的自噬蛋白丰度。
DOI：
10.1016/j.exger.2017.02.075
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发表时间：
2017-06
影响因子：
3.9
通讯作者：
Alway SE
中科院分区：
文献类型：
--
作者：
Takahashi H;Suzuki Y;Mohamed JS;Gotoh T;Pereira SL;Alway SE
We have previously found that Epigallocatechin-3-gallate (EGCg), an abundant catechin in green tea, reduced apoptotic signaling and improved muscle recovery in response to reloading after hindlimb suspension (HS). In this study, we investigated if EGCg altered autophagy signaling in skeletal muscle of old rats in response to HS or reloading after HS. Fischer 344 x Brown Norway inbred rats (age 34 mo.) were given 1 ml/day of purified EGCg (50 mg/kg body weight), or the same sample volume of the vehicle by gavage. One group of animals received HS for 14 days and the second group of rats received 14 days of HS, then the HS was removed and they were allowed to recover by ambulating normally around the cage for two weeks. EGCg decreased a small number of autophagy genes in control muscles, but it increased the expression of other autophagy genes (e.g., ATG16L2, SNCA, TM9SF1, Pink1, PIM-2) and HS did not attenuate these increases. HS increased Beclin1, ATG7 and LC3-II/I protein abundance in hindlimb muscles. Relative to vehicle treatment, EGCg treatment had greater ATG12 protein abundance (35.8%, P<0.05), but decreased Beclin1 protein levels (−101.1%, P<0.05) after HS. However, in reloaded muscles, EGCg suppressed Beclin1 and LC3-II/I protein abundance as compared to vehicle treated muscles. EGCg appeared to “prime” autophagy signaling before and enhance autophagy gene expression and protein levels during unloading in muscles of aged rats, perhaps to improve the clearance of damaged organelles. However, EGCg suppressed autophagy signaling after reloading, potentially to increase the recovery of hindlimb muscles mass and function after loading is restored.
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影响因子：
9
作者：
通讯作者：
--
影响因子：
8.8
作者：
Carnio S;LoVerso F;Baraibar MA;Longa E;Khan MM;Maffei M;Reischl M;Canepari M;Loefler S;Kern H;Blaauw B;Friguet B;Bottinelli R;Rudolf R;Sandri M
通讯作者：
Sandri M
影响因子：
13.3
作者：
Buytaert, E.;Callewaert, G.;Agostinis, P.
通讯作者：
Agostinis, P.
影响因子：
4.8
作者：
Doyle, Alexander;Zhang, Guohua;Li, Yi-Ping
通讯作者：
Li, Yi-Ping
影响因子：
64.8
作者：
Garcia-Prat, Laura;Martinez-Vicente, Marta;Munoz-Canoves, Pura
通讯作者：
Munoz-Canoves, Pura