Observation of high-frequency occurrence of chimeral adventitious shoots in tissue culture from the chimeral tissues of Pelargonium zonale
Observation of high-frequency occurrence of chimeral adventitious shoots in tissue culture from the chimeral tissues of Pelargonium zonale
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DOI：
10.21273/hortsci.40.5.1461
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发表时间：
2005-08
期刊：
影响因子：
1.9
通讯作者：
Ming-yin Li
中科院分区：
文献类型：
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作者：
Ming-yin Li
在天竺葵属中，L1、L2和L3三个独立细胞层中的质体突变可产生具有可见芽颜色差异的质体嵌合体，如GWG（绿色-白色-绿色）和GGW（绿色-绿色-白色）。嵌合体可以用来追踪不同基因型植株地上部发育过程中细胞层间的关系以及突变基因对地上部发育的影响。本研究通过对天竺葵GGW和GWG嵌合体叶柄和节间的组织培养，获得了GGG、GWG、GGW和WWW细胞层组合的不定芽。GGW组织的嵌合不定芽发生率（14.9%）明显高于GWG组织（4.2%）。本试验共获得10.8%的嵌合不定芽，其中8.6%与原外植体类型不同。这一结果表明，至少在外植体的L2和L3中的细胞参与不定芽的再生。再生的芽类型的数量可能取决于与培养基直接接触的细胞的数量和类型。提示混合细胞可用于组织培养生产嵌合体。本文还讨论了体外培养形成嵌合体的三种可能途径。使用的化学名称：TDZ =1-苯基-3-（1，2，3-噻二唑-5-基）脲（噻二隆）; IAA =吲哚-3-乙酸; PVP =聚乙烯吡咯烷酮。
In Pelargonium, the plastid mutation in three independent cell layers L1, L2, and L3, can produce plastid chimeras with visible shoot colour difference such as GWG (green- white-green) and GGW (green-green-white). Chimera can be used to trace the relationship between the cell layers of different genotypes during shoot development and the effect of the mutated genes on shoot development. In this study, we have obtained different adventitious shoots with GGG, GWG, GGW, and WWW combinations of cell layers through tissue culture of petioles and internodes from GGW and GWG chimeras of Pelargonium zonale 'Mrs Pol- lock'. Much higher percentage (14.9%) of chimeral adventitious shoots was obtained from GGW tissues than from GWG tissues (4.2%). Of the 10.8% chimeral adventitious shoots regenerated in this experiment, 8.6% are different from the original type of explants. This result indicated that cells at least in both L2 and L3 of the explants were involved in the regeneration of the adventitious shoots. The number of shoot types regenerated is likely dependent on the number and the type of cells that were in direct contact with the culture medium. It is suggested that the mixed cells can be used to produce the chimera by tissue culture. Three possible ways to form the chimeras in vitro culture were discussed. Chemical names used: TDZ =1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea (Thidiazuron); IAA = Indole-3- acetic acid; PVP = polyvinylpyrrolidone.