Simultaneous in vivo recording of local brain temperature and electrophysiological signals with a novel neural probe
Simultaneous in vivo recording of local brain temperature and electrophysiological signals with a novel neural probe
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DOI：
10.1088/1741-2552/aa60b1
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发表时间：
2017-06-01
影响因子：
4
通讯作者：
Bartho, P.
中科院分区：
文献类型：
--
作者：
Fekete, Z.;Csernai, M.;Bartho, P.
Objective.在正常和病理状态下，温度是神经功能的重要因素，然而，同时监测局部脑温度和神经元活动尚未进行。Approach.在我们的工作中，我们提出了一种可植入的，校准的多模态生物传感器，有利于复杂的调查在皮层和脑深部区域，记录在小鼠神经元群体的多单元活动的热变化。制造的神经探针包含四个电记录站点和一个铂温度传感器灯丝集成在同一探针轴上，距离最近的记录站点30 μ m。在体内研究中提出了同时功能的可行性。探针在麻醉小鼠的丘脑中进行测试，同时操纵动物的核心温度。主要结果。我们获得了多单位和局部场记录沿着局部脑温度的测量，精度为0.14摄氏度。脑温通常跟随核心体温，但也显示出与局部神经活动增加的时期相对应的叠加波动。随着更高电流的应用，我们将局部温度提高了几度，在34-39摄氏度之间没有可观察到的组织损伤。意义所提出的多功能工具的设想，以扩大我们的知识的作用，在皮层和更深的大脑区域的神经元活动的热调制。
Objective. Temperature is an important factor for neural function both in normal and pathological states, nevertheless, simultaneous monitoring of local brain temperature and neuronal activity has not yet been undertaken. Approach. In our work, we propose an implantable, calibrated multimodal biosensor that facilitates the complex investigation of thermal changes in both cortical and deep brain regions, which records multiunit activity of neuronal populations in mice. The fabricated neural probe contains four electrical recording sites and a platinum temperature sensor filament integrated on the same probe shaft within a distance of 30 mu m from the closest recording site. The feasibility of the simultaneous functionality is presented in in vivo studies. The probe was tested in the thalamus of anesthetized mice while manipulating the core temperature of the animals. Main results. We obtained multiunit and local field recordings along with measurement of local brain temperature with accuracy of 0.14 degrees C. Brain temperature generally followed core body temperature, but also showed superimposed fluctuations corresponding to epochs of increased local neural activity. With the application of higher currents, we increased the local temperature by several degrees without observable tissue damage between 34-39 degrees C. Significance. The proposed multifunctional tool is envisioned to broaden our knowledge on the role of the thermal modulation of neuronal activity in both cortical and deeper brain regions.