Molecular detection of Leucocytozoon lovati from probable vectors, black flies (Simuliudae) collected in the alpine regions of Japan
Molecular detection of Leucocytozoon lovati from probable vectors, black flies (Simuliudae) collected in the alpine regions of Japan
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DOI：
10.1007/s00436-008-1183-1
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发表时间：
2009-01-01
影响因子：
2
通讯作者：
Murata, Koichi
中科院分区：
文献类型：
--
作者：
Sato, Yukita;Tamada, Asumi;Murata, Koichi
在日本高山地区，日本岩雷鸟（Lagopus mutus）的宿主鸟类的栖息地，收集了可能的鸟类血液原生动物的节肢动物载体，洛氏白细胞虫。2004 - 2007年，对日本的七个高山地区，旭岳、长岳、立山、次岳、纪仓、北岳和千岳进行了黑蝇收集调查。经形态学鉴定，共采集到490只雌性黑蝇6种，分别为毛足拟黑蝇组（Prosimuplushirtipes group，n = 59）、变拟黑蝇组（穆塔塔，n = 13）、条斑拟黑蝇组（Prosimuplusyezoense，n = 10）、日本蚋（Similiumjaponicum，n = 359）、内田蚋（Similiumuchidai，n = 39）和日本双纹蚋（Twinniajaponensis，n = 10）。从不同种类的黑蝇中提取DNA，用于扩增罗氏白细胞虫线粒体细胞色素B基因的部分序列。四个S japonicum、2株S.在490只黑蝇中，内田黑蝇和毛足黑蝇的巢式PCR检测阳性率为1.6%（8/490）。所有黑蝇的扩增序列与L.以前在日本岩雷鸟中发现的lovati。我们的结果表明，至少有三种黑蝇，即黑蝇。japonicum、日本血吸虫S. Uchidai和P. hirtipes类群是该地区L.岩雷鸟中的lovati。这是日本首次从黑蝇中发现白细胞体。
Probable arthropod vectors of avian blood protozoa, Leucocytozoon lovati, were collected in the alpine regions of Japan, the habitats of the host birds of Japanese rock ptarmigan (Lagopus mutus japonicus). Seven alpine regions of Japan, Asahidake, Chogatake, Tateyama, Jiigatake, Norikura, Kitadake, and Senjyogatake were investigated for black fly collection during 2004 to 2007. The collected 490 insects were morphologically identified as six species of female black flies, including Prosimulium hirtipes group (n = 59), Prosimulium mutata (n = 13), Prosimulium yezoense (n = 10), Similium japonicum (n = 359), Similium uchidai (n = 39), and Twinnia japonensis (n = 10). Extracted DNAs from individual black fly species were utilized for the amplification of the partial mitochondrial cytochrome b gene sequences of Leucocytozoon lovati previously reported. Four S. japonicum, two S. uchidai, and two P. hirtipes group studied were positive for the nested PCR among 490 black flies collected (1.6%; 8/490). All amplified sequences from the black flies were completely identical to those of L. lovati previously detected from Japanese rock ptarmigan. Our results suggest that at least three species of black flies, S. japonicum, S. uchidai, and P. hirtipes group, studied in this area could be regarded as potential vectors for L. lovati in the rock ptarmigan. This is the first detection case of Leucocytozoon from black flies of Japan.