MYCOLIC ACID PATTERNS OF SOME SPECIES OF MYCOBACTERIUM

MYCOLIC ACID PATTERNS OF SOME SPECIES OF MYCOBACTERIUM
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DOI:
10.1007/bf00402005
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发表时间:
1984-01-01
影响因子:
2.8
通讯作者:
MAGNUSSON, M
MAGNUSSON, M
中科院分区:
生物学4区
文献类型:
--
作者:
MINNIKIN, DE;MINNIKIN, SM;MAGNUSSON, M

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一些分枝杆菌属的代表菌株。酸和碱甲醇分解降解。二维薄层色谱法被用来确定这些甲醇裂解物中的分枝菌酸和其他长链组分的模式。由α-,在M. asiaticum,M. bovis、牛黑脉菌M. gastri,M. gordonae、黑脉叶蝉M. kansasii,M. marinum和M.结核病; M.热裂解胆汁也含有较低的MW α。除了这三种酸之外,还有霉菌酸。以M. avium,M. intracellulare、M. nonchromogenicum,M. novum,M. paratuberculosis,M. scrofulaceum、M. termium,M. xenopi和分枝杆菌属MNC 165 α-酮基真菌酸酯伴随有邻羧基真菌酸酯和2-二十烷醇和衍生自蜡酯真菌酸酯的同系物醇。M. fortutuplant和M. giae含有α。和环氧霉菌以及M.猿猴具有非常典型的α模式。“-",α。和酮菌酸。与其他分枝杆菌的数据比较表明,这些分枝菌酸模式的化学分类学意义。
Representative strains of some Mycobacterium spp. were degraded by acid and alkaline methanolysis. Two-dimensional TLC was used to determine the patterns of mycolic acids and other long-chain components in these methanolysates. Patterns composed of .alpha.-, methoxy- and ketomycolates were found in M. asiaticum, M. bovis, M. gastri, M. gordonae, M. kansasii, M. marinum and M. tuberculosis; a representative of M. thermoresistibile also contained lower MW .alpha.''-mycolates in addition to these 3 acids. In representatives of M. avium, M. intracellulare, M. nonchromogenicum, M. novum, M. paratuberculosis, M. scrofulaceum, M. terrae, M. xenopi and Mycobacterium sp. MNC 165 .alpha.- and ketomycolates were accompanied by o-carboxymycolates and 2-eicosanol and homologous alcohols which are derived from wax-ester mycolates. M. fortuitum and M. giae contained .alpha.''- and epoxymycolates and both serovars of M. simiae had a very characteristic pattern of .alpha.''-,.alpha.''- and ketomycolic acids. Comparison with data for other mycobacteria showed the chemotaxonomic significance of these mycolic acid patterns.