Tumor-Penetrating Nanotherapeutics Loading a Near-Infrared Probe Inhibit Growth and Metastasis of Breast Cancer
Tumor-Penetrating Nanotherapeutics Loading a Near-Infrared Probe Inhibit Growth and Metastasis of Breast Cancer
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加载近红外探针的肿瘤穿透纳米疗法可抑制乳腺癌的生长和转移
DOI：
10.1002/adfm.201500772
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发表时间：
2015-05-20
影响因子：
19
通讯作者：
Li, Yaping
中科院分区：
文献类型：
--
作者：
He, Xinyu;Bao, Xiaoyue;Li, Yaping
肿瘤的生长和转移是乳腺癌死亡率高的主要原因。在此，首次报道了一种负载近红外(NIR)探针的深度穿透肿瘤的光热纳米疗法，用于乳腺癌肿瘤生长和转移的潜在光热疗法(PTT)。将亲脂性很强的荧光碳菁染料1，1-dioctadecyl-3，3，3，3-tetramethylindotricarbocyanine碘(DIR)的近红外探针包埋在光热纳米治疗剂中，用于PTT的应用。DIR负载的光热纳米治疗剂(DPN)是均匀的纳米粒子，平均直径为24.5+/-4.1 nm。在808 nm激光照射下，DPN在体外和体内表现出比游离DIR更好的热能产生。DPN联合近红外照射可明显抑制转移性4T1乳腺癌细胞的增殖和迁移活性。此外，DPN还可以诱导肿瘤组织中较高的蓄积，并渗透到肿瘤组织的深层。体内PTT测定表明，DPN联合近红外照射可完全抑制乳腺癌的生长和转移。因此，深穿透性DPN可以为乳腺癌的PTT提供一种有前景的肿瘤进展和转移的治疗策略。
The tumor growth and metastasis is the leading reason for the high mortality of breast cancer. Herein, it is first reported a deep tumor-penetrating photothermal nanotherapeutics loading a near-infrared (NIR) probe for potential photothermal therapy (PTT) of tumor growth and metastasis of breast cancer. The NIR probe of 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR), a lipophilicfluorescent carbocyanine dye with strong light-absorbing capability, is entrapped into the photothermal nanotherapeutics for PTT application. The DiR-loaded photothermal nanotherapeutics (DPN) is homogeneous nanometer-sized particles with the mean diameter of 24.5 +/- 4.1 nm. Upon 808 nm laser irradiation, DPN presents superior production of thermal energy than free DiR both in vitro and in vivo. The cell proliferation and migration activities of metastatic 4T1 breast cancer cells are obviously inhibited by DPN in combination with NIR irradiation. Moreover, DPN can induce a higher accumulation in tumor and penetrate into the deep interior of tumor tissues. The in vivo PTT measurements indicate that the growth and metastasis of breast cancer are entirely inhibited by a single treatment of DPN with NIR irradiation. Therefore, the deep tumor-penetrating DPN can provide a promising strategy for PTT of tumor progression and metastasis of breast cancer.