Nanoparticles based on carboxymethylcellulose-modified rice protein for efficient delivery of lutein
Nanoparticles based on carboxymethylcellulose-modified rice protein for efficient delivery of lutein
复制标题
基于羧甲基纤维素改性大米蛋白的纳米颗粒可有效输送叶黄素
DOI：
10.1039/c9fo02439e
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发表时间：
2020
期刊：
影响因子：
6.1
通讯作者：
Zhu Xue-Mei
中科院分区：
文献类型：
--
作者：
Xu Yu;Ma Xiao-Yu;Gong Wei;Li Xiang;Huang Hai-Bo;Zhu Xue-Mei
叶黄素作为一种天然营养素，对人体健康有很多好处。但其化学稳定性差、生物利用度低限制了叶黄素在食品中的应用。本研究的目的是设计和开发大米蛋白酶水解物（RPH）-羧甲基纤维素（CMC）纳米载体，以有效输送叶黄素。通过在 RPH 与 CMC 的比例为 4 : 1 和 pH 为 6.0 的条件下加热（70 °C，20 分钟）制备 RPH-CMC 纳米颗粒。通过傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）和透射电子显微镜（TEM）对纳米粒子的结构进行了表征。此外，还评估了叶黄素负载大米蛋白纳米颗粒（Lut-R）和叶黄素负载CMC-RPH纳米颗粒（Lut-R-C）的稳定性、体外释放特性、抗增殖和药物吸收。体外释放结果显示，与Lut-R相比，Lut-R-C能有效减缓叶黄素在胃中的释放（19.55±1.71%和8.10±0.41%），并在进入小肠后加速其释放（76.64±1.47%和96.07±0.71%）。此外，RPH-CMC纳米颗粒具有良好的生物相容性，当叶黄素浓度为20 μM时，Lut-R-C纳米颗粒能有效抑制乳腺癌细胞（MCF-7）的增殖，抑制率为70%，且呈剂量依赖性。结果发现，Lut-R-C处理的细胞中叶黄素的摄取率和量始终高于Lut-R处理的细胞。 3 h时Caco-2细胞对Lut-R-C的吸收是Lut-R细胞的90.8倍。该研究为提高叶黄素在食品中的应用提供了新思路。
Lutein, as a natural nutrient, has many benefits for human health. However, its poor chemical stability and low bioavailability limit the application of lutein in foods. The aim of this study is to design and develop rice protease hydrolysate (RPH)–carboxymethylcellulose (CMC) nanocarriers for efficient delivery of lutein. RPH–CMC nanoparticles were prepared by heating at a ratio of RPH to CMC of 4 : 1 and pH of 6.0 (70 °C, 20 min). The structure of the nanoparticles was characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and transmission electron microscopy (TEM). In addition, the stability, in vitro release characteristics, anti-proliferation and drug absorption of lutein-loaded rice protein nanoparticles (Lut-R) and lutein-loaded CMC–RPH nanoparticles (Lut-R-C) were evaluated. The results of in vitro release showed that compared with Lut-R, Lut-R-C could effectively slow down the release of lutein in the stomach (19.55 ± 1.71% and 8.10 ± 0.41%) and accelerate its release after entering the small intestine (76.64 ± 1.47% and 96.07 ± 0.71%). In addition, RPH–CMC nanoparticles had good biocompatibility, and Lut-R-C nanoparticles could effectively inhibit the proliferation of breast cancer cells (MCF-7) with inhibition rate of 70% in a dose-dependent manner when lutein concentration was 20 μM. It was found that lutein uptake rate and amount in Lut-R-C treated cells were always higher than that in Lut-R treated cells. The absorption of Lut-R-C by Caco-2 cells was 90.8 times higher than that of Lut-R cells at 3 h. This study provides a new idea for improving the application of lutein in foods.