Induction of a laccase Lcc9 from Coprinopsis cinerea by fungal coculture and its application on indigo dye decolorization
Induction of a laccase Lcc9 from Coprinopsis cinerea by fungal coculture and its application on indigo dye decolorization
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灰鬼伞菌漆酶Lcc9的真菌共培养诱导及其在靛蓝染料脱色中的应用
DOI：
10.1016/j.biortech.2014.03.116
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发表时间：
2014-06-01
影响因子：
11.4
通讯作者：
Xiao, Yazhong
中科院分区：
文献类型：
--
作者：
Pan, Kai;Zhao, Nannan;Xiao, Yazhong
由灰盖鬼伞冈山7（#130）和孔氏菌W5组成的真菌共培养体系产生的漆酶活性比纯培养提高900倍。从C. cinerea首次通过共培养。对Lcc 9进行纯化、表征，发现其在最适pH 6.5和温度60 ℃下对酚类底物具有高活性。该漆酶在碱性条件下稳定，阳离子和有机溶剂对酶活性影响不大。Lcc 9在2，2 '-连氮基-双（3-乙基苯并噻唑啉-6-磺酸盐）存在下对靛蓝染料表现出脱色能力，在最佳催化条件下，50 U/L酶解1 h后，靛蓝染料的脱色率为75%。这些结果表明，真菌共培养可以激活沉默漆酶基因，Lcc 9的独特性质使其成为特定工业和环境应用的候选者。(C)2014爱思唯尔有限公司版权所有。
A fungal coculture system comprised of Coprinopsis cinerea Okayama 7 (#130) and Gongronella sp. w5 produced 900 times higher laccase activity than that in pure culture. A fungal laccase named Lcc9 was induced from C. cinerea for the first time by coculture. Lcc9 was purified, characterized, and found to have high activity toward phenolic substrates at the optimum pH of 6.5 and temperature of 60 degrees C. The laccase was stable at alkaline pH values, and its activity was not significantly affected by cations and organic solvents. Lcc9 showed decolorization capability toward indigo dye in the presence of 2,2'-azino-bis(3-ethylbenzothazoline-6-sulfonate), with 75% of indigo was decolorized by 50 U/L enzyme after 1 h of incubation under optimal catalytic conditions. These results showed that fungal coculture could active silent laccase gene, and the unusual properties make Lcc9 a candidate for specific industrial and environmental applications. (C) 2014 Elsevier Ltd. All rights reserved.