Micro cell encapsulation and its hydrogel-beads production using microfluidic device
Micro cell encapsulation and its hydrogel-beads production using microfluidic device
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DOI：
10.1007/s00542-006-0291-z
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发表时间：
2007-05-01
影响因子：
2.1
通讯作者：
Kotera, Hidetoshi
中科院分区：
文献类型：
--
作者：
Shintaku, Hirofumi;Kuwabara, Takeo;Kotera, Hidetoshi
在本文中，我们提出了一种细胞包封和水凝胶珠生产方法，使用微滴形成在微通道中。与传统的液滴挤出法和乳化法相比，该微流控装置制备的水凝胶珠具有更小的粒径和更窄的粒径分布。基于流体动力学分析，分析了流速和微通道壁面对微通道流动的影响。结果表明，微通道的壁面效应对液滴的直径和水凝胶珠的形状有很大的影响。
In this paper, we propose a cell encapsulation and hydrogel-beads production method using droplet formation in a microchannel. The hydrogel-beads produced by the microfluidic device developed here have smaller diameter and narrower distribution in their diameter compared to the conventional method, such as the droplet extrusion and the emulsification. The effects of the flow velocity and microchannel wall were analyzed based on fluid dynamical analysis. The results revealed that the wall effect of the microchannel strongly affected to the diameter of the droplet and the shape of the hydrogel-beads.