Whole blood flow cytometry measurements of in vivo platelet activation in critically-Ill patients are influenced by variability in blood sampling techniques
Whole blood flow cytometry measurements of in vivo platelet activation in critically-Ill patients are influenced by variability in blood sampling techniques
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DOI：
10.1016/j.thromres.2011.11.031
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发表时间：
2012-06-01
影响因子：
7.5
通讯作者：
Weyrich, Andrew S.
中科院分区：
文献类型：
--
作者：
Rondina, Matthew T.;Grissom, Colin K.;Weyrich, Andrew S.
简介：流式细胞术通常用于测量危重患者的体内血小板活化。在血液采样技术，这可能会混淆这些测量的变异性，仍然很差的characteristic.Materials和方法：血小板活化进行流式细胞术测定116例危重患者的动脉和静脉血。我们确定了如何在血管采样点，处理时间，血小板计数的变异性影响水平的血小板-单核细胞聚集体（PMA），PAC-1结合（糖蛋白（GP）IIbIIIa），和P-选择素（P-SEL）expression.Results：水平的PMA，但不是PAC-1结合或P-SEL的表达，显着受血管采样点的变异性。从动脉血管抽取的全血中的平均PMA水平比静脉血高约60%（16.2 +/- 1.8% vs. 10.7 +/-1.2%，p
Introduction: Flow cytometry is often used to measure in vivo platelet activation in critically-ill patients. Variability in blood sampling techniques, which may confound these measurements, remains poorly characterized.Materials and Methods: Platelet activation was measured by flow cytometry performed on arterial and venous blood from 116 critically-ill patients. We determined how variability in vascular sampling site, processing times, and platelet counts influenced levels of platelet-monocyte aggregates (PMA), PAC-1 binding (for glycoprotein (GP) IIbIIIa), and P-selectin (P-SEL) expression.Results: Levels of PMA, but not PAC-1 binding or P-SEL expression, were significantly affected by variability in vascular sampling site. Average PMA levels were approximately 60% higher in whole blood drawn from an arterial vessel compared to venous blood (16.2 +/- 1.8% vs. 10.7 +/- 1.2%, p