Guard cell wall:: immunocytochemical detection of polysaccharide components
Guard cell wall:: immunocytochemical detection of polysaccharide components
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DOI：
10.1093/jexbot/53.371.1067
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发表时间：
2002-05-01
影响因子：
6.9
通讯作者：
Rodríguez-García, MI
中科院分区：
文献类型：
--
作者：
Majewska-Sawka, A;Münster, A;Rodríguez-García, MI
甜菜叶片保卫细胞壁的组成采用组织化学染色和细胞壁抗原免疫细胞化学检测方法，对细胞壁抗原进行了研究。并与表皮细胞壁和叶肉细胞壁进行了比较。用单克隆抗体对叶切片进行检测，发现果胶、α-（1 -> 2）-半乳糖、β-（1 -> 3）-葡聚糖和阿拉伯半乳糖蛋白质的末端岩藻糖残基连接，揭示了保卫细胞的几个特定特征。JIM 7识别的果胶表位均匀分布在壁中，而JIM 5识别的果胶在壁本身中没有发现，但在表皮层中丰富。大量的分子轴承终端岩藻糖主要位于腹侧保卫细胞壁。在这些细胞的背壁，以及在路面和叶肉细胞的壁中检测到的量要小得多。在保卫细胞质膜附近观察到这些化合物的明显积累，而在邻近细胞表面的细胞壁区域中标记很少。胼胝质的存在清楚地标志着最近形成的，非常年轻的保卫细胞之间的腹壁。胼胝质也出现在一些成熟的细胞壁中，在那里它被看作是点状沉积物，可能反映了保卫细胞的特定生理状态。大量的阿拉伯半乳聚糖蛋白沉积在角质层内，并且在叶的其他组织中也检测到少量的这些蛋白聚糖。保卫细胞壁的组织化学和免疫细胞化学结构进行了讨论，根据其多种功能，其中大部分涉及细胞大小和形状的变化。
The composition of guard cell walls in sugar beet leaves (Beta vulgaris L.) was studied by using histochemical staining and immunocytochemical detection of cell wall antigens. The findings were compared with those in the walls of epidermal and mesophyll cells. Probing of leaf sections with monoclonal antibodies against pectins, terminal fucosyl residues linked alpha-(1 --> 2) to galactose, beta-(1 --> 3)-glucans and arabinogalactan-proteins revealed several specific features of guard cells. Pectic epitopes recognized by JIM7 were homogeneously distributed in the wall, whereas pectins recognized by JIM5 were not found in the walls themselves, but were abundant in the cuticular layer. Large amounts of molecules bearing terminal fucose were located predominantly in ventral and lateral guard cell walls. Much smaller amounts were detected in dorsal walls of these cells, as well as in the walls of pavement and mesophyll cells. Conspicuous accumulation of these compounds was observed in the vicinity of the guard cell plasmalemma, whereas labelling was scarce in the areas of the wall adjacent to the cell surface. The presence of callose clearly marked the ventral wall between the recently formed, very young guard cells. Callose also appeared in some mature walls, where it was seen as punctate deposits that probably reflected a specific physiological state of the guard cells. Large amounts of arabinogalactan-proteins were deposited within the cuticle, and smaller amounts of these proteoglycans were also detected in other tissues of the leaf. The histochemical and immunocytochemical structure of the guard cell wall is discussed in the light of its multiple functions, most of which involve changes in cell size and shape.